PMID- 11314345
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20151119
IS  - 1462-6446 (Print)
IS  - 1462-6446 (Linking)
VI  - 3
IP  - 1
DP  - 2000 May
TI  - The expression kinetics of myogenin in facial muscle denervation.
PG  - 7-11
AB  - OBJECTIVE: To study the expression kinetics of myogenin in long-term denervated 
      facial muscle and to explore the possibility of gene therapy for facial muscle 
      paralysis with myogenin gene. MATERIALS AND METHODS: In 48 New Zealand rabbits, 
      buccal muscle paralysis of one side was produced by excision of 1 cm segment 
      buccal branch of the facial nerve. The opposite side served as controls. The 
      animals were sacrificed and the buccal muscles of both sides were removed for 
      examination at 1 day, 3 days, 1 week, 2 weeks, 1 month, 2 months, 4 months, and 6 
      months after the initial operation. The myogenin expression in denervated and 
      innervated buccal muscles was analyzed by Western blot. Satellite cell 
      proliferation was detected with proliferating cell nuclear antigen (PCNA) 
      analysis. Muscle nucleic acid concentration was determined through acridine 
      orange (AO) staining method. RESULTS: Myogenin expression increased to the 
      highest level at 3 days after denervation, thereafter it gradually decreased. The 
      Western blotting signal for myogenin intensified again after 1 month and at 4 
      months. In contrast, the highest expression of the controls (innervated muscles) 
      were observed at 1 month. These changes in myogenin expression in denervated 
      buccal muscles were consistent with the change in satellite cell proliferation 
      and in muscle nucleic acid concentration. CONCLUSION: Myogenin protein expression 
      in longterm facial muscle denervation is closely associated with satellite cell 
      regeneration. Myogenin may promote satellite cell differentiation, and therefore 
      may improve the treatment of facial paralysis.
FAU - Xiao, R
AU  - Xiao R
AD  - Department of Oral and Maxillofacial Surgery, School of Stomatology, Beijing 
      Medical University (BMU), 38 Bai Shi Qiao Road, Haidian District, Beijing, 
      100081, P. R. China.
FAU - Yu, G
AU  - Yu G
FAU - Jia, H
AU  - Jia H
FAU - Cai, Z
AU  - Cai Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Chin J Dent Res
JT  - The Chinese journal of dental research
JID - 100892845
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Myogenin)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
MH  - Acridine Orange
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation
MH  - Cell Division
MH  - DNA/analysis
MH  - Facial Muscles/*innervation/pathology
MH  - Facial Nerve/*metabolism/pathology/surgery
MH  - Fluorescent Dyes
MH  - Follow-Up Studies
MH  - Gene Expression Regulation
MH  - Male
MH  - Microscopy, Confocal
MH  - Muscle Denervation
MH  - Myogenin/*genetics/metabolism
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - RNA/analysis
MH  - Rabbits
EDAT- 2001/04/21 10:00
MHDA- 2001/06/08 10:01
CRDT- 2001/04/21 10:00
PHST- 2001/04/21 10:00 [pubmed]
PHST- 2001/06/08 10:01 [medline]
PHST- 2001/04/21 10:00 [entrez]
PST - ppublish
SO  - Chin J Dent Res. 2000 May;3(1):7-11.