PMID- 11314892
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190831
IS  - 0303-6979 (Print)
IS  - 0303-6979 (Linking)
VI  - 28
IP  - 4
DP  - 2001 Apr
TI  - Longitudinal evaluation of GCF MMP-3 and TIMP-1 levels as prognostic factors for 
      progression of periodontitis.
PG  - 353-9
AB  - BACKGROUND: To determine whether matrix metalloproteinase-3 (MMP-3) and tissue 
      inhibitor of metalloproteinases-1 (TIMP-1) in gingival crevicular fluid (GCF) 
      could serve as prognostic factors for the progression of periodontitis, we 
      monitored GCF MMP-3 and TIMP-1 and periodontal status of selected sites in 40 
      medically healthy subjects over a 6-month period. METHOD: Clinical measurements 
      including gingival index (GI), plaque index, bleeding on probing, suppuration, 
      probing depth (PD), attachment loss (AL), and GCF samples were taken from 2 
      healthy sites (including sites with gingival recession, GI=0 PD < or =3 mm; AL < 
      or =2 mm) and 2 periodontitis sites (GI > or =1; PD > or =5 mm; AL > or =3 mm) of 
      each patient at baseline, 3-month and 6-month visits by means of sterile paper 
      strips. GCF levels of MMP-3 and TIMP-1 were determined by sandwich ELISA assays. 
      RESULTS: The mean amounts of MMP-3 and TIMP-1 in diseased sites were 
      significantly higher than in healthy sites (p<0.0001). Significantly higher GCF 
      levels of MMP-3 and TIMP-1 were found at progressing sites than in nonprogressing 
      periodontitis sites (0.001<p<0.01). A progressing site was defined as a site 
      which had > or =2 mm loss of attachment during 6- month study period. GCF levels 
      of MMP-3 were highly correlated with clinical measurements taken at baseline, 
      3-month and 6-month visits (p<0.001). TIMP-1 levels were only moderately 
      correlated with probing depth and attachment level (p<0.01). Step-wise multiple 
      regression analysis was performed to construct models for the prediction of 
      probing depth and attachment loss increases. The most parsimonious regression 
      models which had the best R2 values included the following variables and 
      accounted for the indicated % of variability. The regression model for the 
      prediction of probing depth increase included MMP-3, smoking pack-years, TIMP-1 
      and accounted for 53% of the variability. The best model for the prediction of 
      attachment loss increase included MMP-3, smoking pack-years, age, TIMP-1 and 
      explained 59% of the variability. CONCLUSION: These data indicate that sites with 
      high GCF levels of MMP-3 and TIMP-1 are at significantly greater risk for 
      progression of periodontitis.
FAU - Alpagot, T
AU  - Alpagot T
AD  - University of the Pacific School of Dentistry, San Francisco, CA 94115, USA. 
      talpagot@uop-edu
FAU - Bell, C
AU  - Bell C
FAU - Lundergan, W
AU  - Lundergan W
FAU - Chambers, D W
AU  - Chambers DW
FAU - Rudin, R
AU  - Rudin R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Periodontol
JT  - Journal of clinical periodontology
JID - 0425123
RN  - 0 (Biomarkers)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Analysis of Variance
MH  - Biomarkers/analysis
MH  - Dental Plaque Index
MH  - Disease Progression
MH  - Female
MH  - Follow-Up Studies
MH  - Forecasting
MH  - Gingival Crevicular Fluid/*enzymology
MH  - Gingival Hemorrhage/classification/enzymology
MH  - Gingival Recession/enzymology
MH  - Gingivitis/enzymology
MH  - Humans
MH  - Longitudinal Studies
MH  - Male
MH  - Matrix Metalloproteinase 3/*analysis
MH  - Middle Aged
MH  - Periodontal Attachment Loss/classification/enzymology
MH  - Periodontal Index
MH  - Periodontal Pocket/classification/enzymology
MH  - Periodontitis/*enzymology/physiopathology
MH  - Prognosis
MH  - Protease Inhibitors/*analysis
MH  - Regression Analysis
MH  - Risk Factors
MH  - Smoking/physiopathology
MH  - Suppuration
MH  - Tissue Inhibitor of Metalloproteinase-1/*analysis
EDAT- 2001/04/21 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/04/21 10:00
PHST- 2001/04/21 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/04/21 10:00 [entrez]
AID - 10.1034/j.1600-051x.2001.028004353.x [doi]
PST - ppublish
SO  - J Clin Periodontol. 2001 Apr;28(4):353-9. doi: 
      10.1034/j.1600-051x.2001.028004353.x.