PMID- 11315944
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20061115
IS  - 1122-6714 (Print)
IS  - 1122-6714 (Linking)
VI  - 103
IP  - 4 Suppl 1
DP  - 1998
TI  - Confocal laser fluorescence scanning microscopy of the oolemma distribution of 
      monoclonal antibodies against mouse and human egg surface membrane.
PG  - 129-36
AB  - Monoclonal antibodies were raised against zona-free mouse oocytes by intrasplenic 
      immunizations. Antibodies, which detected antigen epitopes in unfertilized but 
      not in fertilized mouse eggs (fertilization-specific antibodies) and which in 
      addition recognized epitopes in unfertilized human oocytes (mouse-human 
      cross-reacting antibodies) were used to visualize the distribution of their 
      corresponding antigens by confocal laser fluoresence scanning microscopy (CLFSM). 
      We found that among 26 antibodies tested, two ones showed a patch pattern. Since 
      a patchy distribution of cell membrane molecules indicates the presence of 
      integral proteins with ability to transduce signals to the cytoplasm, we suggest 
      that the method here applied will select for those oolemma molecules that could 
      be involved in the fusion of sperm cells.
FAU - Jin, M
AU  - Jin M
AD  - Department of Human Anatomy, University of Uppsala, Sweden.
FAU - Hjortberg, M
AU  - Hjortberg M
FAU - Hamren, J
AU  - Hamren J
FAU - Sundstrom, P
AU  - Sundstrom P
FAU - Nilsson, B O
AU  - Nilsson BO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Ital J Anat Embryol
JT  - Italian journal of anatomy and embryology = Archivio italiano di anatomia ed 
      embriologia
JID - 9612303
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Epitopes/immunology
MH  - Fertilization/immunology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Confocal
MH  - Ovum/immunology/*ultrastructure
MH  - Vitelline Membrane/immunology/*ultrastructure
MH  - Zona Pellucida/immunology/*ultrastructure
EDAT- 2001/04/24 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/04/24 10:00
PHST- 2001/04/24 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/04/24 10:00 [entrez]
PST - ppublish
SO  - Ital J Anat Embryol. 1998;103(4 Suppl 1):129-36.