PMID- 11316057
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20161124
IS  - 0231-5882 (Print)
IS  - 0231-5882 (Linking)
VI  - 19
IP  - 3
DP  - 2000 Sep
TI  - Calcium store depletion induced by mitochondrial uncoupling in prostatic cells.
PG  - 265-78
AB  - The effects of mitochondrial uncoupling on the calcium homeostasis of prostatic 
      cells were investigated using the prostatic cancer cell line LNCaP and indo-1 
      spectrofluorimetry. Carbonyl cyanide m-chloro-phenylhydrazone (CCCP) was used as 
      uncoupler. Resting LNCaP cells responded to CCCP by a biphasic increase in 
      [Ca2+]i. The first phase of increase which corresponded to the release of a 
      mitochondrial CCCP-sensitive Ca2+ store was followed by a second increase phase 
      consisting of Ca2+ influx through the plasma membrane. The relationship between 
      the CCCP- and the InsP3-sensitive stores was investigated using thapsigargin 
      (TG). The release part of the Ca2+ response to TG was reduced in a time-dependent 
      manner by previous exposure of the cells to CCCP, suggesting that CCCP also acts 
      on non-mitochondrial stores. Our results show that CCCP releases Ca2+ from both 
      mitochondrial and non-mitochondrial stores in prostatic cells. The possible 
      mechanisms of these effects are discussed.
FAU - Vaur, S
AU  - Vaur S
AD  - Laboratoire de Neurophysiologie, CNRS UMR 5543, Universite Victor 
      Segalen-Bordeaux II, France.
FAU - Sartor, P
AU  - Sartor P
FAU - Dufy-Barbe, L
AU  - Dufy-Barbe L
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Gen Physiol Biophys
JT  - General physiology and biophysics
JID - 8400604
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Oligomycins)
RN  - 0 (Uncoupling Agents)
RN  - 11118-72-2 (antimycin)
RN  - 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone)
RN  - 642-15-9 (Antimycin A)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - N18RMK75W1 (indo-1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Antimycin A/*analogs & derivatives/pharmacology
MH  - Calcium/*metabolism
MH  - Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
MH  - Cell Membrane/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes/pharmacology
MH  - Humans
MH  - Indoles/pharmacology
MH  - Male
MH  - Mitochondria/*metabolism
MH  - Oligomycins/pharmacology
MH  - Prostatic Neoplasms/*metabolism
MH  - Spectrometry, Fluorescence
MH  - Thapsigargin/pharmacology
MH  - Time Factors
MH  - Tumor Cells, Cultured
MH  - Uncoupling Agents/*pharmacology
EDAT- 2001/04/24 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/04/24 10:00
PHST- 2001/04/24 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/04/24 10:00 [entrez]
PST - ppublish
SO  - Gen Physiol Biophys. 2000 Sep;19(3):265-78.