PMID- 11316749
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20161124
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 142
IP  - 5
DP  - 2001 May
TI  - 17beta-estradiol treatment decreases steroidogenic enzyme messenger ribonucleic 
      acid levels in the rainbow trout testis.
PG  - 1841-8
AB  - In fish, estrogens are well known for their involvement in ovarian 
      differentiation and have been shown to be very potent feminizing agents when 
      administrated in vivo during early development. However, the mechanism of action 
      of exogenous estrogens is poorly understood. We report here on the feminizing 
      effects of estrogen treatment on the testicular levels of some steroidogenic 
      enzyme messenger RNAs [mRNAs; cholesterol side-chain cleavage (P450scc), 
      17-hydroxylase/lyase (P450c17), 3beta-hydroxysteroid dehydrogenase (3betaHSD), 
      11beta-hydroxylase (P45011beta), and aromatase (P450aro)] in the rainbow trout, 
      Oncorhynchus mykiss. Treatment was carried out by dietary administration of 
      17beta-estradiol (E(2); dosage of 20 mg/kg diet) to a genetically all male 
      population. Steroidogenesis in the differentiating testis was demonstrated to be 
      strongly altered by E(2), as this treatment resulted in considerable decrease in 
      P450c17, 3betaHSD, and P45011beta mRNAs after only 10 days of treatment. In 
      contrast, P450scc and P450aro mRNA levels were unaffected by E(2), with P450scc 
      mRNA levels remaining unaltered and P450aro not stimulated by this feminizing 
      estrogen treatment. To better characterize this E(2) effect, the same treatment 
      was applied on postdifferentiating males, and roughly the same expression pattern 
      was detected with a considerable decrease in testicular P450c17, 3betaHSD, and 
      P45011beta mRNAs and a significant, but reduced, decrease in P450scc mRNA. In the 
      interrenal, these steroidogenic enzyme mRNAs were not significantly affected by 
      this E(2) treatment, except for a slight, but significant, decrease in P450scc 
      mRNA. These results clearly demonstrate that estrogens have profound effects on 
      testicular steroidogenesis and that they are acting specifically on the testis by 
      decreasing mRNA steady state levels of many steroidogenic enzyme genes. The 
      decrease in P45011beta mRNA, and thus inhibition of the synthesis of testicular 
      11-oxygenated androgens, may be an important step required for the active 
      feminization of these genetic males.
FAU - Govoroun, M
AU  - Govoroun M
AD  - Institut National de la Recherche Agronomique, Laboratoire Institut National de 
      la Recherche Agronomique-Station Commune de Recherches en Ichtyophysiologie, 
      Biodiversite et Environement, Campus de Beaulieu, 35042 Rennes Cedex, France.
FAU - McMeel, O M
AU  - McMeel OM
FAU - Mecherouki, H
AU  - Mecherouki H
FAU - Smith, T J
AU  - Smith TJ
FAU - Guiguen, Y
AU  - Guiguen Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 1.14.15.4 (Steroid 11-beta-Hydroxylase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/genetics
MH  - Animals
MH  - Aromatase/genetics
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics
MH  - Estradiol/*pharmacology
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Male
MH  - Oncorhynchus mykiss
MH  - RNA, Messenger/*analysis
MH  - Steroid 11-beta-Hydroxylase/genetics
MH  - Steroid 17-alpha-Hydroxylase/biosynthesis
MH  - Steroid Hydroxylases/genetics
MH  - Steroids/*biosynthesis
MH  - Testis/*drug effects/enzymology
EDAT- 2001/04/24 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/04/24 10:00
PHST- 2001/04/24 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/04/24 10:00 [entrez]
AID - 10.1210/endo.142.5.8142 [doi]
PST - ppublish
SO  - Endocrinology. 2001 May;142(5):1841-8. doi: 10.1210/endo.142.5.8142.