PMID- 11316850
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20210511
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 12
IP  - 5
DP  - 2001 May
TI  - Chemokine and chemokine receptor expression during initiation and resolution of 
      immune complex glomerulonephritis.
PG  - 919-931
LID - 10.1681/ASN.V125919 [doi]
AB  - Chemokines participate in leukocyte infiltration, which plays a major role in 
      glomerular injury during immune complex glomerulonephritis (IC-GN). Because 
      target cell expression of chemokine receptors (CCR) is thought to mediate 
      leukocyte migration, the expression pattern of chemokines and CCR in a model of 
      IC-GN was examined. The transient course and predominant glomerular pathology of 
      this model allows the examination of both the induction and resolution phases of 
      IC-GN. GN was induced in mice by daily apoferritin injection for 2 wk. Urine 
      samples and kidneys were obtained at 1, 2, and 4 wk. Albuminuria was noted at 2 
      wk, but resolved after 4 wk. This was associated with glomerular IC deposits and 
      mesangial proliferation. Glomerular macrophage infiltration was prominent at 1 
      and 2 wk, which resolved at 4 wk. Expression of monocyte chemoattractant 
      protein-1 (MCP-1) and RANTES mRNA was upregulated at week 1 and decreased to 
      control levels at weeks 2 and 4. The expression was localized to glomeruli by in 
      situ hybridization and immunohistochemistry. The mRNA of CCR1, CCR2, and CCR5 but 
      not CCR3 or CCR4 were upregulated at week 1 and decreased at weeks 2 and 4. 
      Expression of CCR5 was located to the glomerulus by in situ hybridization and 
      quantitative reverse transcription-PCR of isolated glomeruli. In summary, in a 
      model of transient IC-GN, MCP-1 and RANTES and their receptors CCR1, CCR2, and 
      CCR5 are expressed early and are already downregulated at the peak of proteinuria 
      and leukocyte infiltration. Resolution of glomerulonephritis is associated with a 
      return to baseline of chemokine and CCR expression. Therefore, it is concluded 
      that glomerular MCP-1 and RANTES production directs circulating leukocytes that 
      express CCR1, CCR2, and CCR5 into the glomerulus. After initiating GN, MCP-1 and 
      RANTES and their receptors are readily downregulated.
FAU - Anders, Hans-Joachim
AU  - Anders HJ
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Vielhauer, Volker
AU  - Vielhauer V
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Kretzler, Matthias
AU  - Kretzler M
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Cohen, Clemens D
AU  - Cohen CD
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Segerer, Stephan
AU  - Segerer S
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Luckow, Bruno
AU  - Luckow B
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Weller, Lars
AU  - Weller L
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
FAU - Grone, Hermann-Josef
AU  - Grone HJ
AD  - Division of Experimental Pathology, German Cancer Research Center, Heidelberg, 
      Germany.
FAU - Schlondorff, Detlef
AU  - Schlondorff D
AD  - Nephrological Center, Medical Policlinic, Ludwig-Maximilians-University Munich, 
      Munich, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Chemokines)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Chemokine)
RN  - 9013-31-4 (Apoferritins)
SB  - IM
MH  - Animals
MH  - Apoferritins/administration & dosage
MH  - Base Sequence
MH  - Chemokines/*genetics/*metabolism
MH  - DNA Primers/genetics
MH  - Female
MH  - Gene Expression
MH  - Glomerulonephritis/etiology/*genetics/*immunology/pathology
MH  - Immune Complex Diseases/etiology/*genetics/*immunology/pathology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Leukocytes/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Chemokine/*genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/04/24 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/04/24 10:00
PHST- 2001/04/24 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/04/24 10:00 [entrez]
AID - 12/5/919 [pii]
AID - 10.1681/ASN.V125919 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2001 May;12(5):919-931. doi: 10.1681/ASN.V125919.