PMID- 11316993
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20190718
IS  - 0269-9370 (Print)
IS  - 0269-9370 (Linking)
VI  - 15
IP  - 5
DP  - 2001 Mar 30
TI  - Beta amyloid precursor protein and patterns of HIV p24 immunohistochemistry in 
      different brain areas of AIDS patients.
PG  - 571-5
AB  - OBJECTIVES: To evaluate the correlation between immunohistochemical positive 
      patterns (globular and filamentous structures) of beta-amyloid precursor protein 
      (beta-APP), used as a marker of axonal damage, and the different distribution of 
      HIV p24 antigens, in three different brain areas of AIDS patients. METHODS: 
      Eighteen AIDS patients with HIV-related brain lesions were included in the study. 
      Forty-nine sections from basal ganglia, frontal cortex and hippocampus were 
      selected. After microwave oven pre-treatment, the sections were incubated with 
      anti-HIV p24 and anti-beta-APP monoclonal antibodies; the reactions were 
      developed with peroxidase/3,3'diaminobenzidine. The positivity was graded by 
      semi-quantitative scores. Double immunohistochemical staining was used to 
      evaluate the co-localization of the antigens. RESULTS: HIV p24 
      immunohistochemistry was positive in 44 of 49 sections (89%), with a prevalence 
      of interstitial positive cells and positive microglial nodules in 27 and 13 
      sections respectively. beta-APP-positive structures were demonstrated in 23 of 44 
      sections (52%) with HIV-related lesions, and were absent from the five sections 
      without viral expression. Globular and filamentous lesions were observed in 21 of 
      23 sections and 10 of 23 lesions respectively. Moreover, a high grade of globular 
      type lesion was related to an elevated presence of diffuse interstitial HIV 
      p24-positive cells in basal ganglia; double immunohistochemical reactions 
      demonstrated the co-localization of beta-APP globules and HIV p24 antigens. 
      CONCLUSIONS: The data obtained confirm the coexpression of beta-APP and viral 
      antigens in particular areas of the brain with HIV-related lesions; there is a 
      strict correlation between beta-APP globules (indicating chronic cerebral damage) 
      and the interstitial pattern of HIV p24 immunohistochemistry.
FAU - Nebuloni, M
AU  - Nebuloni M
AD  - Pathology Unit, 'Ospedale Civile', Vimercate, the Pathologhy Unit, 'L. Sacco' 
      Institute of Medical Science, University of Milan, Italy.
FAU - Pellegrinelli, A
AU  - Pellegrinelli A
FAU - Ferri, A
AU  - Ferri A
FAU - Bonetto, S
AU  - Bonetto S
FAU - Boldorini, R
AU  - Boldorini R
FAU - Vago, L
AU  - Vago L
FAU - Grassi, M P
AU  - Grassi MP
FAU - Costanzi, G
AU  - Costanzi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Biomarkers)
RN  - 0 (HIV Core Protein p24)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/epidemiology/*metabolism/pathology/virology
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Basal Ganglia/*metabolism/pathology/virology
MH  - Biomarkers
MH  - Frontal Lobe/*metabolism/pathology/virology
MH  - HIV Core Protein p24/*metabolism
MH  - *HIV-1
MH  - Hippocampus/*metabolism/pathology/virology
MH  - Humans
MH  - Italy/epidemiology
MH  - Retrospective Studies
EDAT- 2001/04/24 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/04/24 10:00
PHST- 2001/04/24 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/04/24 10:00 [entrez]
AID - 10.1097/00002030-200103300-00005 [doi]
PST - ppublish
SO  - AIDS. 2001 Mar 30;15(5):571-5. doi: 10.1097/00002030-200103300-00005.