PMID- 11319133
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 64
IP  - 5
DP  - 2001 May
TI  - Retinoid receptors involved in the effects of retinoic acid on rat testis 
      development.
PG  - 1307-14
AB  - We have previously shown that retinoic acid (RA) is able to act on the 
      development of Leydig, Sertoli, and germ cells in the testis in culture (Livera 
      et al., Biol Reprod 2000; 62:1303-1314). To identify which receptors mediate 
      these effects, we have now added selective agonists and antagonists of retinoic 
      acid receptors (RARs) or retinoid X receptors (RXRs) in the same organotypic 
      culture system. The RAR alpha agonist mimicked most of the effects of RA on the 
      cultured fetal or neonatal testis, whereas the RAR beta, gamma, and pan RXR 
      agonists did not. The RAR alpha agonist decreased the testosterone production, 
      the number of gonocytes, and the cAMP response to FSH of fetal testis explanted 
      at 14.5 days postconception (dpc). The RAR alpha agonist disorganized the cords 
      of the 14.5-dpc cultured testis and increased the cord diameter in cultured 
      3-days-postpartum (dpp) testis in the same way as RA. All these RA effects could 
      be reversed by an RAR alpha antagonist and were unchanged by an RAR beta/gamma 
      antagonist. The RAR beta agonist, however, increased Sertoli cell proliferation 
      in the 3-dpp testis in the same way as RA, and this effect was blocked by an RAR 
      beta antagonist. The RAR gamma and the pan RXR agonists had no selective effect. 
      These results suggest that all the effects of RA on development of the fetal and 
      neonatal testis are mediated via RAR alpha, except for its effect on Sertoli cell 
      proliferation, which involves RAR beta.
FAU - Livera, G
AU  - Livera G
AD  - Universite Paris 7 and INSERM-INRA U 418, Tour 33/43, case 7126, 75251 Paris 
      Cedex 05, France.
FAU - Rouiller-Fabre, V
AU  - Rouiller-Fabre V
FAU - Habert, R
AU  - Habert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Retinoids)
RN  - 0 (Transcription Factors)
RN  - 3XMK78S47O (Testosterone)
RN  - 5688UTC01R (Tretinoin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Division/drug effects
MH  - Culture Techniques
MH  - Cyclic AMP/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Luteinizing Hormone/pharmacology
MH  - Male
MH  - Morphogenesis/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Retinoic Acid/agonists/antagonists & inhibitors/*physiology
MH  - Retinoid X Receptors
MH  - Retinoids/pharmacology
MH  - Sertoli Cells/drug effects
MH  - Testis/*embryology/*growth & development/metabolism
MH  - Testosterone/biosynthesis
MH  - Transcription Factors/agonists/antagonists & inhibitors/physiology
MH  - Tretinoin/*pharmacology
EDAT- 2001/04/25 10:00
MHDA- 2001/08/17 10:01
CRDT- 2001/04/25 10:00
PHST- 2001/04/25 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/04/25 10:00 [entrez]
AID - 10.1095/biolreprod64.5.1307 [doi]
PST - ppublish
SO  - Biol Reprod. 2001 May;64(5):1307-14. doi: 10.1095/biolreprod64.5.1307.