PMID- 11319609
OWN - NLM
STAT- MEDLINE
DCOM- 20051201
LR  - 20161124
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 8
IP  - 3
DP  - 2001 Mar
TI  - A20 zinc finger protein inhibits TNF-induced apoptosis and stress response early 
      in the signaling cascades and independently of binding to TRAF2 or 14-3-3 
      proteins.
PG  - 265-72
AB  - A20 zinc finger protein is a negative regulator of tumor necrosis factor 
      (TNF)-induced signaling pathways leading to apoptosis, stress response and 
      inflammation. A20 has been shown to bind to TNF-receptor-associated factor 2 
      (TRAF2) and 14-3-3 chaperone proteins. Our data indicate that the zinc finger 
      domain of A20 is sufficient and that neither TRAF2 nor 14-3-3 binding is 
      necessary for the inhibitory effects of A20. Mutations in the 14-3-3 binding site 
      of A20 did, however, result in a partial cleavage of A20 protein suggesting that 
      14-3-3 chaperone proteins may stabilize A20. Furthermore, we show that A20 acts 
      early in TNF-induced signaling cascades blocking both TNF-induced rapid 
      activation of c-Jun N-terminal kinase and processing of the receptor-associated 
      caspase-8. Taken together our data indicate that the zinc finger domain of A20 
      contains all necessary functional domains required for the inhibition of TNF 
      signaling and that A20 may function at the level of the receptor signaling 
      complex.
FAU - Lademann, U
AU  - Lademann U
AD  - Apoptosis Laboratory, Institute of Cancer Biology, Danish Cancer Society, 
      Strandboulevarden 49, DK-2100 Copenhagen, Denmark.
FAU - Kallunki, T
AU  - Kallunki T
FAU - Jaattela, M
AU  - Jaattela M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Caspase Inhibitors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FADD protein, human)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Proteins)
RN  - 0 (RNAIII inhibiting peptide)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor Receptor-Associated Peptides and Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 3.4.19.12 (TNFAIP3 protein, human)
RN  - EC 3.4.19.12 (Tumor Necrosis Factor alpha-Induced Protein 3)
RN  - EC 3.4.22.- (CASP8 protein, human)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - 14-3-3 Proteins/genetics/*metabolism
MH  - Adaptor Proteins, Signal Transducing/biosynthesis
MH  - Apoptosis/*drug effects/physiology
MH  - Binding Sites
MH  - Blotting, Western
MH  - Breast Neoplasms/genetics/metabolism/pathology
MH  - Caspase 8
MH  - Caspase Inhibitors
MH  - Caspases/metabolism
MH  - Cell Line, Tumor
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins
MH  - Fas-Associated Death Domain Protein
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/biosynthesis
MH  - Intracellular Signaling Peptides and Proteins
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Nuclear Proteins
MH  - Oligopeptides/biosynthesis
MH  - Protein Binding
MH  - Proteins/genetics/metabolism
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - TNF Receptor-Associated Factor 2/biosynthesis/*metabolism
MH  - Transfection
MH  - Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism
MH  - Tumor Necrosis Factor alpha-Induced Protein 3
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/pharmacology
MH  - *Zinc Fingers
EDAT- 2001/04/25 10:00
MHDA- 2005/12/13 09:00
CRDT- 2001/04/25 10:00
PHST- 2000/08/22 00:00 [received]
PHST- 2000/10/23 00:00 [revised]
PHST- 2000/10/30 00:00 [accepted]
PHST- 2001/04/25 10:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2001/04/25 10:00 [entrez]
AID - 10.1038/sj.cdd.4400805 [doi]
PST - ppublish
SO  - Cell Death Differ. 2001 Mar;8(3):265-72. doi: 10.1038/sj.cdd.4400805.