PMID- 11319647
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20191025
VI  - 25
IP  - 4
DP  - 2001 Apr
TI  - Genetic variation in the stress protein hsp70-2 gene is highly associated with 
      obesity.
PG  - 462-6
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) expression is increased in 
      adipose tissue of both rodent models of obesity and obese humans. It has 
      therefore been considered as a candidate gene for obesity. Several studies have 
      indeed shown statistical evidence of linkage between obesity and the chromosomal 
      region encompassing the TNF-alpha gene, suggesting that TNF-alpha and/or a nearby 
      gene (eg hsp70 gene) is involved in the onset and progression of weight gain. We 
      designed a case-controlled study to investigate the potential association of 
      polymorphism of the TNF-alpha and that of a stress protein (hsp70-2) with 
      obesity. METHODS: We used the polymerase chain reaction and restriction enzyme 
      digestion to characterize the variation of the TNF-alpha promoter region and that 
      of the hsp70-2 gene in 343 unrelated Tunisian patients with obesity and 174 
      healthy control subjects. RESULTS: Analysis of the -308 TNF-alpha polymorphism in 
      patients with obesity and in control subjects did not reveal an association 
      between TNF-alpha alleles and obesity. In contrast, polymorphism analysis of the 
      hsp70-2 gene in patients with obesity demonstrated highly significant differences 
      in genotypic distribution of this bi-allelic locus compared to the control 
      subject group. Homozygosity for one hsp70-2 allele was highly associated with 
      obesity (r2=7.12; P<10(-6)). CONCLUSION: Tunisian persons carrying the P2/P2 
      genotype of the hsp70-2 gene may have an increased risk of obesity.
FAU - Chouchane, L
AU  - Chouchane L
AD  - Laboratoire d'Immuno-Oncologie Moleculaire, Faculte de Medecine de Monastir, 
      Monastir, Tunisia. lotfi.chouchane@planet.tn
FAU - Danguir, J
AU  - Danguir J
FAU - Beji, C
AU  - Beji C
FAU - Bouassida, K
AU  - Bouassida K
FAU - Camoin, L
AU  - Camoin L
FAU - Sfar, H
AU  - Sfar H
FAU - Gabbouj, S
AU  - Gabbouj S
FAU - Strosberg, A D
AU  - Strosberg AD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Obes Relat Metab Disord
JT  - International journal of obesity and related metabolic disorders : journal of the 
      International Association for the Study of Obesity
JID - 9313169
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSPA2 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Female
MH  - *Genetic Variation
MH  - Genotype
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Male
MH  - Obesity/etiology/*genetics
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Restriction Mapping
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2001/04/25 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/04/25 10:00
PHST- 2000/01/05 00:00 [received]
PHST- 2000/09/04 00:00 [revised]
PHST- 2000/10/02 00:00 [accepted]
PHST- 2001/04/25 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/04/25 10:00 [entrez]
AID - 10.1038/sj.ijo.0801545 [doi]
PST - ppublish
SO  - Int J Obes Relat Metab Disord. 2001 Apr;25(4):462-6. doi: 10.1038/sj.ijo.0801545.