PMID- 11320311
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20190605
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 57
IP  - Pt 5
DP  - 2001 May
TI  - Expression, crystallization and preliminary characterization of methylmalonyl 
      coenzyme A epimerase from Propionibacterium shermanii.
PG  - 706-8
AB  - Methylmalonyl-CoA epimerase (MMCE) is an enzyme that interconverts the R and S 
      epimers of methylmalonyl-CoA in the pathway that links propionyl-CoA with 
      succinyl-CoA. This is used for both biosynthetic and degradative processes, 
      including the breakdown of odd-numbered fatty acids and some amino acids. The 
      enzyme has been expressed in Escherichia coli both as the native enzyme and as 
      its selenomethionine (SeMet) derivative. Crystals of both forms have been 
      obtained by vapour diffusion using monomethylether PEG 2000 as precipitant. The 
      native MMCE crystals are orthorhombic, with unit-cell parameters a = 56.0, b = 
      114.0, c = 156.0 A, and the SeMet-MMCE crystals are monoclinic, with unit-cell 
      parameters a = 43.6, b = 78.6, c = 89.4 A, beta = 92.0 degrees; both diffract to 
      better than 2.8 A resolution.
FAU - McCarthy, A A
AU  - McCarthy AA
AD  - School of Biological Sciences, University of Auckland, Auckland, New Zealand.
FAU - Baker, H M
AU  - Baker HM
FAU - Shewry, S C
AU  - Shewry SC
FAU - Kagawa, T F
AU  - Kagawa TF
FAU - Saafi, E
AU  - Saafi E
FAU - Patchett, M L
AU  - Patchett ML
FAU - Baker, E N
AU  - Baker EN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010424
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - EC 5.1.- (Racemases and Epimerases)
RN  - EC 5.1.99.1 (methylmalonyl-coenzyme A racemase)
SB  - IM
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Propionibacterium/*enzymology
MH  - Racemases and Epimerases/*chemistry/genetics
EDAT- 2001/04/26 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/04/26 10:00
PHST- 2001/01/17 00:00 [received]
PHST- 2001/01/30 00:00 [accepted]
PHST- 2001/04/26 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/04/26 10:00 [entrez]
AID - S0907444901002050 [pii]
AID - 10.1107/s0907444901002050 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2001 May;57(Pt 5):706-8. doi: 
      10.1107/s0907444901002050. Epub 2001 Apr 24.