PMID- 11321541
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20191104
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 3
IP  - 2
DP  - 2001 Feb
TI  - In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating 
      traits for microbial populations in a biological phosphorus removal process.
PG  - 110-22
AB  - Polyphosphate- and polyhydroxyalkanoate (PHA)-accumulating traits of predominant 
      microorganisms in an efficient enhanced biological phosphorus removal (EBPR) 
      process were investigated systematically using a suite of non-culture-dependent 
      methods. Results of 16S rDNA clone library and fluorescence in situ hybridization 
      (FISH) with rRNA-targeted, group-specific oligonucleotide probes indicated that 
      the microbial community consisted mostly of the alpha- (9.5% of total cells), 
      beta- (41.3%) and gamma- (6.8%) subclasses of the class Proteobacteria, 
      Flexibacter-Cytophaga (4.5%) and the Gram-positive high G+C (HGC) group (17.9%). 
      With individual phylogenetic groups or subgroups, members of Candidatus 
      Accumulibacter phosphatis in the beta-2 subclass, a novel HGC group closely 
      related to Tetrasphaera spp., and a novel gamma-proteobacterial group were the 
      predominant populations. Furthermore, electron microscopy with energy-dispersive 
      X-ray analysis was used to validate the staining specificity of 
      4,6-diamino-2-phenylindole (DAPI) for intracellular polyphosphate and revealed 
      the composition of polyphosphate granules accumulated in predominant bacteria as 
      mostly P, Ca and Na. As a result, DAPI and PHA staining procedures could be 
      combined with FISH to identify directly the polyphosphate- and PHA-accumulating 
      traits of different phylogenetic groups. Members of Accumulibacter phosphatis and 
      the novel gamma-proteobacterial group were observed to accumulate both 
      polyphosphate and PHA. In addition, one novel rod-shaped group, closely related 
      to coccus-shaped Tetrasphaera, and one filamentous group resembling Candidatus 
      Nostocoidia limicola in the HGC group were found to accumulate polyphosphate but 
      not PHA. No cellular inclusions were detected in most members of the 
      alpha-Proteobacteria and the Cytophaga-Flavobacterium group. The diversified 
      functional traits observed suggested that different substrate metabolisms were 
      used by predominant phylogenetic groups in EBPR processes.
FAU - Liu, W T
AU  - Liu WT
AD  - Graduate Institute of Environmental Engineering, National Central University, 
      Chungli, Taiwan. liuwt@cc.ncu.edu.tw
FAU - Nielsen, A T
AU  - Nielsen AT
FAU - Wu, J H
AU  - Wu JH
FAU - Tsai, C S
AU  - Tsai CS
FAU - Matsuo, Y
AU  - Matsuo Y
FAU - Molin, S
AU  - Molin S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Polyphosphates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Acetates/metabolism
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Bacteria/classification/genetics/*isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - Cloning, Molecular
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Phosphorus/*metabolism
MH  - Phylogeny
MH  - Polyphosphates/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/analysis/*microbiology
EDAT- 2001/04/26 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/04/26 10:00
PHST- 2001/04/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/04/26 10:00 [entrez]
AID - 10.1046/j.1462-2920.2001.00164.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2001 Feb;3(2):110-22. doi: 10.1046/j.1462-2920.2001.00164.x.