PMID- 11324519 OWN - NLM STAT- MEDLINE DCOM- 20010517 LR - 20061115 IS - 0371-0874 (Print) IS - 0371-0874 (Linking) VI - 50 IP - 1 DP - 1998 Feb TI - [The different responses to anoxia in cultured CA1 and DG neurons from newborn rats]. PG - 61-6 AB - Tissue culture from hippocampal CA1 or dentate gyrus (DG) region was established on the basis of previous neuronal culture technique. The viability, intracellular calcium concentration and brain-derived neurotrophic factor (BDNF) mRNA expression of the two kinds of neurons after anoxia were observed in the counting in the confocal microscopic field and in situ hybridization. It is found that DG neurons are not only more resistant to anoxia, but also have a stronger ability to keep calcium homeostasis and to express BDNF mRNA than CA1 neurons. FAU - Yao, H AU - Yao H AD - Institute of Basic Medical Sciences, Beijing 100850. FAU - Huang, Y H AU - Huang YH FAU - Liu, Z W AU - Liu ZW FAU - Wan, Q AU - Wan Q FAU - Ding, A S AU - Ding AS FAU - Zhao, B AU - Zhao B FAU - Fan, M AU - Fan M FAU - Wang, F Z AU - Wang FZ LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Sheng Li Xue Bao JT - Sheng li xue bao : [Acta physiologica Sinica] JID - 20730130R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics MH - Cell Hypoxia MH - Cell Survival MH - Cells, Cultured MH - Dentate Gyrus/cytology/metabolism/*physiology MH - Hippocampus/cytology/*physiology MH - Neurons/cytology MH - RNA, Messenger/biosynthesis/genetics MH - Rats MH - Rats, Wistar EDAT- 2001/04/28 10:00 MHDA- 2001/05/18 10:01 CRDT- 2001/04/28 10:00 PHST- 2001/04/28 10:00 [pubmed] PHST- 2001/05/18 10:01 [medline] PHST- 2001/04/28 10:00 [entrez] PST - ppublish SO - Sheng Li Xue Bao. 1998 Feb;50(1):61-6.