PMID- 11324756
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20191025
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 22
IP  - 2
DP  - 2001 Mar
TI  - Large envelope glycoprotein and nucleocapsid protein of equine arteritis virus 
      (EAV) induce an immune response in Balb/c mice by DNA vaccination; strategy for 
      developing a DNA-vaccine against EAV-infection.
PG  - 187-99
AB  - Equine arteritis virus (EAV) is a member of the Arteriviridae family, that 
      includes lactate dehydrogenase-elevating virus (LDV), porcine reproductive and 
      respiratory syndrome virus (PRRSV), and simian haemorrhagic fever virus (SHFV). 
      Equine arteritis is a contagious disease of horses and is spread via respiratory 
      or reproductive tract. The objective of the present study is to evaluate the 
      possibility for developing a model system for prevention horses against an EAV 
      infection by DNAvaccination. A cDNA bank from the RNA of EAV was established. 
      This gene library contains the translation unit of the EAV open reading frames 
      (ORF) 1 to 7. The identity of the cDNA was confirmed by nucleotide sequence 
      analysis. Using this defined EAV cDNA gene library the cDNA sequence of the viral 
      ORFs were molecularly cloned into the corresponding sites of well characterized 
      and powerful expression vectors (pCR3.1, pDisplay, and/or pcDNA3.1/HisC). The 
      capability of these recombinant plasmids expressing the gene products of the 
      individual viral ORFs 3 to 5, and 7 in induction of an immune response in mouse 
      system was investigated. The Balb/c mice (ten mice per assay) were inoculated 
      with the DNA of the constructed expression vectors harboring and expressing the 
      EAV cDNA of the viral ORFs. The Balb/c mice were injected with about 100 microg 
      DNA diluted in 100 microl PBS. The DNA was injected subcutaneously and into the 
      tibialis cranialis muscle (Musculus gastrocnemius). The mice were boosted 3 to 5 
      times with the same quantities of DNA and under the same conditions at about two 
      week intervals. Control mice received the same amount of parental expression 
      vectors via an identical route and frequency. The pre- and post-vaccinated sera 
      of the individual animals were screened by neutralization tests (NT). 
      Neutralizing antibodies against EAV were detected when the animals were 
      inoculated with the DNA of the expression vectors harboring cDNA of the EAV ORFs 
      5 and 7. Highest NT-titers were observed when the animals were administered with 
      the cDNA of ORF 5 and/or with the cDNA of the neutralization determinants of EAV 
      that is located on the N-terminal ectodomain of the gene product of ORF 5 between 
      the amino acid positions 1-121. These results obtained from these studies 
      justified proofing the capability of the EAV cDNA sequences of the viral genes 
      including ORFs 5 and 7 in the autologous animal system horse.
FAU - Tobiasch, E
AU  - Tobiasch E
AD  - Institut fur Medizinische Virologie der Universitat Heidelberg, Federal Republic 
      of Germany.
FAU - Kehm, R
AU  - Kehm R
FAU - Bahr, U
AU  - Bahr U
FAU - Tidona, C A
AU  - Tidona CA
FAU - Jakob, N J
AU  - Jakob NJ
FAU - Handermann, M
AU  - Handermann M
FAU - Darai, G
AU  - Darai G
FAU - Giese, M
AU  - Giese M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (EAV nucleocapsid protein, Equine arteritis virus)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein L, equine arteritis virus)
SB  - IM
MH  - Animals
MH  - Arterivirus Infections/immunology/prevention & control
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Equartevirus/*genetics/immunology
MH  - Gene Expression
MH  - Genes, Viral
MH  - Genetic Vectors
MH  - Horses
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nucleocapsid Proteins/*genetics/immunology
MH  - Open Reading Frames
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vaccination
MH  - Vaccines, DNA/genetics/*immunology
MH  - Viral Envelope Proteins/*genetics/immunology
MH  - Viral Vaccines/genetics/*immunology
EDAT- 2001/04/28 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/04/28 10:00
PHST- 2001/04/28 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/04/28 10:00 [entrez]
AID - 10.1023/a:1008175525254 [doi]
PST - ppublish
SO  - Virus Genes. 2001 Mar;22(2):187-99. doi: 10.1023/a:1008175525254.