PMID- 11324758
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20240328
IS  - 0920-8569 (Print)
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 22
IP  - 2
DP  - 2001 Mar
TI  - Comparison of the 5' leader sequences of North American isolates of reference and 
      field strains of porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 209-17
AB  - The 5' leader is documented to be an important regulatory element in many (+) 
      ssRNAvirus genome. To understand the significance of the 5' leader RNA of PRRSV, 
      we determined the complete leader sequences of fifteen different North American 
      strains of PRRSV and predicted their secondary structures. Viruses analysed 
      included three reference strains and nine field strains originating from 
      different geographic locations. To further examine the leader region, one of the 
      field strains was adapted to grow in tissue culture, and three clones were 
      isolated. We also predicted the secondary structures of two European strains 
      based on their published sequences. The predicted RNA secondary structures of the 
      leader sequences suggested the existence of three conserved domains formed by the 
      5' region of the leader among the North American strains, two of which were 
      conserved in the European strains. A variable structural domain was predicted 
      from the 3' region of the leader sequences of the North American strains, where 
      all tissue culture-adapted isolates were characterized by a stem-loop while field 
      isolates were characterized by an internal bulge within the stem-loop.
FAU - Tan, C
AU  - Tan C
AD  - Institute of Molecular Agrobiology, National University of Singapore, Singapore. 
      jimmykwang@ima.org.sg
FAU - Chang, L
AU  - Chang L
FAU - Shen, S
AU  - Shen S
FAU - Liu, D X
AU  - Liu DX
FAU - Kwang, J
AU  - Kwang J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry
MH  - Animals
MH  - Base Sequence
MH  - Electronic Data Processing
MH  - Molecular Sequence Data
MH  - North America
MH  - *Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - RNA, Viral/*chemistry
MH  - Swine
PMC - PMC7088843
EDAT- 2001/04/28 10:00
MHDA- 2001/11/03 10:01
PMCR- 2020/03/23
CRDT- 2001/04/28 10:00
PHST- 2001/04/28 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/04/28 10:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 320376 [pii]
AID - 10.1023/a:1008179726163 [doi]
PST - ppublish
SO  - Virus Genes. 2001 Mar;22(2):209-17. doi: 10.1023/a:1008179726163.