PMID- 11326317
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190607
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 3
IP  - 1
DP  - 2001 Jan-Feb
TI  - Evidence of chromosomal instability in prostate cancer determined by spectral 
      karyotyping (SKY) and interphase fish analysis.
PG  - 62-9
AB  - The way in which cytogenetic aberrations develop in prostate cancer (CaP) is 
      poorly understood. Spectral karyotype (SKY) analysis of CaP cell lines has shown 
      that they have unstable karyotypes and also have features associated with 
      chromosomal instability (CIN). To accurately determine the incidence of de novo 
      structural and numerical aberrations in vitro in CaP, we performed SKY analysis 
      of three independent clones derived from one representative cell line, DU145. The 
      frequent generation of new chromosomal rearrangements and a wide variation in the 
      number of structural aberrations within two to five passages suggested that this 
      cell line exhibited some of the features associated with a CIN phenotype. To 
      study numerical cell-to-cell variation, chromosome 8 aneusomy was assessed in the 
      LNCaP, DU145, and PC-3 cell lines and a patient cohort of 15 CaP primary tumors 
      by interphase fluorescence in situ hybridization (FISH). This analysis showed 
      that a high frequency of numerical alteration affecting chromosome 8 was present 
      in both in vitro and in CaP tissues. In comparison to normal controls, the 
      patient cohort had a statistically significant (P<.05), greater frequency of 
      cells with one and three centromere 8 copies. These data suggest that a CIN-like 
      process may be contributing towards the generation of de novo numerical and 
      structural chromosome abnormalities in CaP.
FAU - Beheshti, B
AU  - Beheshti B
AD  - Department of Laboratory Medicine and Pathobiology, University of Toronto, 
      Toronto, ON, Canada.
FAU - Park, P C
AU  - Park PC
FAU - Sweet, J M
AU  - Sweet JM
FAU - Trachtenberg, J
AU  - Trachtenberg J
FAU - Jewett, M A
AU  - Jewett MA
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - *Chromosome Disorders
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Karyotyping/*methods
MH  - Male
MH  - Ploidies
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Tumor Cells, Cultured
PMC - PMC1505026
EDAT- 2001/04/28 10:00
MHDA- 2001/06/22 10:01
PMCR- 2001/01/01
CRDT- 2001/04/28 10:00
PHST- 2000/10/23 00:00 [received]
PHST- 2000/11/23 00:00 [accepted]
PHST- 2001/04/28 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/04/28 10:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 0125a [pii]
AID - 10.1038/sj.neo.7900125 [doi]
PST - ppublish
SO  - Neoplasia. 2001 Jan-Feb;3(1):62-9. doi: 10.1038/sj.neo.7900125.