PMID- 11326934
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20131121
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 34
IP  - 5
DP  - 1999 May
TI  - [Study on decidual and villous cells apoptosis and its control gene in medical 
      abortion].
PG  - 281-3
AB  - OBJECTIVE: To study relationship of decidual and villous cells apoptosis and its 
      control gene B-cell lymphoma/leukemia-2(bcl-2), bcl-2 associated X protein (bax) 
      with medical abortion. METHODS: Forty four women who volunteered to terminate 
      their pregnancy were divided into 3 group: mifepristone group (exp. 1, n = 15), 
      mifepristone with misoprostol group (exp. II, n = 14) and control group (n = 15). 
      The cell cycles of DNA of the decidual and villous cells stained with Propidium 
      Iodide (PI) were analyzed and bcl-2 and bax gene protein labeled with 
      immuno-fluorescence were detected by flow cytometry. RESULTS: The percentage of 
      decidual cells apoptosis were (14.0 +/- 3.9)%, (22.9 +/- 6.3)%, (5.3 +/- 9.3)% 
      and of villous apoptosis were (10.9 +/- 2.8)%, (13.9 +/- 4.7)%, (4.6 +/- 3.0)% in 
      exp. I, exp. II and control group respectively. The apoptotic percentages in 
      decidual and villous cells of exp. I and exp. II groups were significantly higher 
      than that of control (P < 0.01-0.001). On DNA histogram, apoptosis peaks appeared 
      in all the groups. The decidual cell apoptosis of exp. II groups was 
      significantly higher than that of exp. I group (P < 0.01). Both decidual 
      apoptotic percentages of exp. I and exp. II groups were significantly higher than 
      that of villous (P < 0.01). Decidual bcl-2/bax ratio were 1.4 +/- 0.7, 0.9 +/- 
      0.3, 1.4 +/- 0.7 and villous bcl-2/bax ratio were 1.0 +/- 0.4, 0.8 +/- 0.3, 1.4 
      +/- 0.6 in the 3 groups respectively. Both decidual and villous bcl-2/bax ratio 
      of exp. II group was significantly lower than that of control (P < 0.05-0.01) 
      which was in consistent with apoptosis. CONCLUSIONS: These data indicated that 
      mifepristone with misocprostol for terminating human early pregnancy might be 
      realized by inducing decidual and villous cells excessive apoptosis. The descent 
      of bcl-2/bax ratio could be one of the factors of inducing decidual and villous 
      apoptosis.
FAU - Li, R
AU  - Li R
AD  - Second Hospital, Hebei Medical University, Shijiazhuang 050000.
FAU - Wang, Z
AU  - Wang Z
FAU - Wu, R
AU  - Wu R
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Abortifacient Agents, Nonsteroidal)
RN  - 0 (Abortifacient Agents, Steroidal)
RN  - 0 (BAX protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0E43V0BB57 (Misoprostol)
RN  - 320T6RNW1F (Mifepristone)
SB  - IM
MH  - Abortifacient Agents, Nonsteroidal
MH  - *Abortifacient Agents, Steroidal
MH  - Abortion, Induced/methods
MH  - Adult
MH  - Apoptosis/*drug effects
MH  - Chorionic Villi/*pathology
MH  - Decidua/*cytology
MH  - Female
MH  - Gene Expression Regulation
MH  - *Genes, bcl-2
MH  - Humans
MH  - *Mifepristone
MH  - Misoprostol
MH  - Pregnancy
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - bcl-2-Associated X Protein
EDAT- 2001/05/01 10:00
MHDA- 2001/06/02 10:01
CRDT- 2001/05/01 10:00
PHST- 2001/05/01 10:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2001/05/01 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 1999 May;34(5):281-3.