PMID- 11326967
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20131121
IS  - 1001-0939 (Print)
IS  - 1001-0939 (Linking)
VI  - 21
IP  - 4
DP  - 1998 Apr
TI  - [Effects of smoking on the lipid peroxides antioxidation and alpha 
      1-antitrypsin].
PG  - 218-20
AB  - OBJECTIVE: Detecting free radicals and alpha 1-antitrypsin (alpha 1-AT) from 
      healthy smoker's blood to study the action of free radicals in smoking induced 
      diseases. METHOD: Thiobarbituric acid method (TBA), RIA, 
      5-5'-dithio-2-dinitrobenzoicacid (DTNB) method, Ellman method and enzyme linked 
      immunosorbent assay were used to detect the lipid peroxides (LPO), superoxide 
      dismutase (SOD), glutathione peroxidase (GSH-px), glutathione (GSH) and alpha 
      1-AT. RESULT: In the blood of nonsmokers, LPO was 4.9 +/- 2.5 mumol/L, SOD was 
      878 +/- 302 ng/g Hb, GSH-px was 120 +/- 35 U, GSH was 3.4 +/- 0.7 mmol/L, alpha 
      1-AT was 75 +/- 11 mg/L, in the blood of smokers, they were 6.1 +/- 3.0 mumol/L, 
      780 +/- 282 ng/g Hb, 112 +/- 34 U, 3.1 +/- 0.8 mmol/L, 71 +/- 15 mg/L 
      respectively. The LPO content in the blood of smokers was significant higher than 
      that of nonsmokers (P < 0.01), but the SOD GSH-px GSH and alpha 1-AT contents 
      measured was significant lower (P < 0.05 or P < 0.01). CONCLUSION: The increase 
      of oxidation and decrease of antioxidation, activity of alpha 1-AT and imbalance 
      of protease and antiprotease might play important roleg in smoking induced 
      diseases.
FAU - Zhao, Y X
AU  - Zhao YX
AD  - Respiratory Disease Institution, Chinese Medical University, Shenyang 110001.
FAU - Yu, R J
AU  - Yu RJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Jie He He Hu Xi Za Zhi
JT  - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal 
      of tuberculosis and respiratory diseases
JID - 8712226
RN  - 0 (Lipid Peroxides)
RN  - 0 (alpha 1-Antitrypsin)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adult
MH  - Female
MH  - Glutathione/blood
MH  - Glutathione Peroxidase/blood
MH  - Humans
MH  - Lipid Peroxides/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Smoking/*blood
MH  - Superoxide Dismutase/blood
MH  - alpha 1-Antitrypsin/*metabolism
EDAT- 2001/05/01 10:00
MHDA- 2001/05/26 10:01
CRDT- 2001/05/01 10:00
PHST- 2001/05/01 10:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/05/01 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Jie He He Hu Xi Za Zhi. 1998 Apr;21(4):218-20.