PMID- 11328373
OWN - NLM
STAT- MEDLINE
DCOM- 20010510
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 102
IP  - 4
DP  - 2001 Apr
TI  - Functional and phenotypic characterization of distinct porcine dendritic cells 
      derived from peripheral blood monocytes.
PG  - 396-404
AB  - Dendritic cells (DCs) are bone marrow-derived antigen-presenting cells that have 
      an exquisite capacity to interact with T cells and modulate their responses. 
      Little is known about porcine DCs despite the fact that they represent an 
      important target in strategies that are aimed at modulating resistance to 
      infection in pigs and may be of major importance in transplantation biology. We 
      generated immature monocyte-derived porcine dendritic cells (MoDCs) directly from 
      adherent peripheral blood cells treated with porcine granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cells were 
      observed via electron microscopy and their phenotype was characterized using 
      monoclonal antibodies. The functionality of the porcine MoDCs was demonstrated 
      showing that the cells were capable of different specialized functions relevant 
      to antigen capture and were potent stimulators in a primary allo-mixed leucocyte 
      reaction. Treatment of the MoDCs with porcine cell line-derived necrotic factors 
      resulted in the phenotypic and functional maturation of MoDCs. We confirmed also 
      that monocyte-derived DCs were differentially regulated by cytokines, showing 
      that transforming growth factor-beta1 (TGF-beta1) is able to redirect monocytic 
      precursors into the differentiation pathway of Langerhans' cells presenting 
      typical Birbeck granules. Interestingly, and in contrast to the human and murine 
      model, we showed that the monocyte-derived porcine Langerhans'-type cells (MoLCs) 
      were much more potent activators of allogeneic T cells than MoDCs obtained 
      without TGF-beta1.
FAU - Paillot, R
AU  - Paillot R
AD  - Discovery Research, Merial, 254 rue Marcel Merieux BP 7009, 69 342 Cedex 07, Lyon 
      France.
FAU - Laval, F
AU  - Laval F
FAU - Audonnet, J C
AU  - Audonnet JC
FAU - Andreoni, C
AU  - Andreoni C
FAU - Juillard, V
AU  - Juillard V
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/*immunology/ultrastructure
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/immunology
MH  - Immunophenotyping
MH  - Interleukin-4/immunology
MH  - Langerhans Cells/immunology
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Monocytes/*immunology
MH  - Swine/*immunology
MH  - Transforming Growth Factor beta/immunology
MH  - Transforming Growth Factor beta1
PMC - PMC1783196
EDAT- 2001/05/01 10:00
MHDA- 2001/05/22 10:01
PMCR- 2002/04/01
CRDT- 2001/05/01 10:00
PHST- 2001/05/01 10:00 [pubmed]
PHST- 2001/05/22 10:01 [medline]
PHST- 2001/05/01 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - imm1200 [pii]
AID - 10.1046/j.1365-2567.2001.01200.x [doi]
PST - ppublish
SO  - Immunology. 2001 Apr;102(4):396-404. doi: 10.1046/j.1365-2567.2001.01200.x.