PMID- 11329374
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 114
IP  - Pt 10
DP  - 2001 May
TI  - Nuclear dispositions of subtelomeric and pericentromeric chromosomal domains 
      during meiosis in asynaptic mutants of rye (Secale cereale L.).
PG  - 1875-82
AB  - The nuclear dispositions of subtelomeric and pericentromeric domains in pollen 
      mother cells (PMCs) were tracked during meiosis in wildtype and two asynaptic 
      mutants of rye (Secale cereale L.) by means of fluorescence in situ hybridization 
      (FISH). Homozygotes for sy1 and sy9 non-allelic mutations form axial elements 
      during leptotene of male meiosis, but fail to form synaptonemal complexes. 
      Consequently, recombination is severely impaired, and high univalency is observed 
      at metaphase I. Simultaneous FISH with pSc200 subtelomeric tandem repeat and CCS1 
      centromeric sequence revealed that at pre-meiotic interphase the two domains are 
      in a bipolar Rabl orientation in both the PMCs and tapetal cells. At the onset of 
      meiotic prophase, the subtelomeric regions in PMCs of wildtype and sy9 cluster 
      into a typical bouquet conformation. The timing of this event in rye is 
      comparable with that in wheat, and is earlier than that observed in other 
      organisms, such as maize, yeast and mammals. This arrangement is retained until 
      later in leptotene and zygotene when the pericentromeric domains disperse and the 
      subtelomeric clusters fragment. The mutant phenotype of sy9 manifests itself 
      during leptotene to zygotene, when the pericentromeric regions become distinctly 
      more distended than in wildtype, and largely fail to pair during zygotene. This 
      indicates that difference in the nature or timing of chromosome condensation in 
      this region is the cause or consequence of asynapsis. By contrast, sy1 fails to 
      form comparable aggregates of subtelomeric regions at leptotene in only half of 
      the nuclei studied. Instead, two to five aggregates are formed that fail to 
      disperse at later stages of meiotic prophase. In addition, the pericentromeric 
      regions disperse prematurely at leptotene and do not associate in pairs at any 
      subsequent stage. It is supposed that the sy1 mutation could disrupt the nuclear 
      disposition of centromeres and telomeres at the end of pre-meiotic interphase, 
      which could cause, or contribute to, its asynaptic phenotype.
FAU - Mikhailova, E I
AU  - Mikhailova EI
AD  - Dept of Genetics, Saint-Petersburg State University, Russia.
FAU - Sosnikhina, S P
AU  - Sosnikhina SP
FAU - Kirillova, G A
AU  - Kirillova GA
FAU - Tikholiz, O A
AU  - Tikholiz OA
FAU - Smirnov, V G
AU  - Smirnov VG
FAU - Jones, R N
AU  - Jones RN
FAU - Jenkins, G
AU  - Jenkins G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Cell Nucleus/*genetics/metabolism
MH  - Centromere/*genetics/metabolism
MH  - DNA, Plant/analysis
MH  - Epistasis, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Metaphase/physiology
MH  - Microscopy, Confocal
MH  - Mutation/physiology
MH  - Phenotype
MH  - Pollen/genetics
MH  - Secale/*genetics
MH  - Synaptonemal Complex/*genetics/metabolism
MH  - Telomere/*genetics/metabolism
EDAT- 2001/05/01 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/05/01 10:00
PHST- 2001/05/01 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/05/01 10:00 [entrez]
AID - 10.1242/jcs.114.10.1875 [doi]
PST - ppublish
SO  - J Cell Sci. 2001 May;114(Pt 10):1875-82. doi: 10.1242/jcs.114.10.1875.