PMID- 11330673
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20031114
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 65
IP  - 3
DP  - 2001 Mar
TI  - Oxidative stress by visible light irradiation suppresses immunoglobulin 
      production in mouse spleen lymphocytes.
PG  - 593-8
AB  - In this study, we attempted to induce the oxidative stress in mouse spleen 
      lymphocytes with visible light irradiation and examined the effects of lipid 
      peroxidation on immunoglobulin (Ig) production. The spleen lymphocytes were 
      isolated from 8-week-old male balb/c mice and irradiated with 300 W visible 
      light. When the cells were cultured for 72 hr, Ig contents in culture 
      supernatants were decreased gradually by irradiation for over 30 min. The cell 
      viability was also lowered by the irradiation. Intracellular phosphatidylcholine 
      hydroperoxide (PCOOH) levels and thiobarbituric acid-reactive substances (TBARS) 
      values in culture supernatants were measured as indices of lipid peroxidation and 
      we found that Ig production by mouse spleen lymphocytes was suppressed 
      accompanied with the progress of peroxidation of intracellular phospholipids. 
      Cell membrane fluidity was also significantly decreased, but the intracellular Ig 
      level was not changed in the irradiated cells. These results suggest that the 
      peroxidation of intracellular lipids is a cause of the suppression of Ig 
      production by mouse spleen lymphocytes via lowering cell viability and 
      suppressing Ig synthesis and secretion.
FAU - Miyazaki, Y
AU  - Miyazaki Y
AD  - Institute of Applied Biological Chemistry, Department of Bioscience and 
      Biotechnology, Graduate School of Kyushu University, Fukuoka, Japan.
FAU - Yamasaki, M
AU  - Yamasaki M
FAU - Mishima, H
AU  - Mishima H
FAU - Mansho, K
AU  - Mansho K
FAU - Tachibana, H
AU  - Tachibana H
FAU - Yamada, K
AU  - Yamada K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 1406-18-4 (Vitamin E)
SB  - IM
MH  - Animals
MH  - Cell Membrane/radiation effects
MH  - Cells, Cultured
MH  - Immunoglobulin G/*biosynthesis
MH  - Immunoglobulin M/*biosynthesis
MH  - Intracellular Fluid/metabolism
MH  - Light
MH  - *Lipid Peroxidation
MH  - Lymphocytes/drug effects/immunology/*radiation effects
MH  - Male
MH  - Membrane Fluidity/radiation effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Oxidative Stress
MH  - Spleen/immunology
MH  - Vitamin E/metabolism/pharmacology
EDAT- 2001/05/02 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/05/02 10:00
PHST- 2001/05/02 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/05/02 10:00 [entrez]
AID - 10.1271/bbb.65.593 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2001 Mar;65(3):593-8. doi: 10.1271/bbb.65.593.