PMID- 11331040
OWN - NLM
STAT- MEDLINE
DCOM- 20010802
LR  - 20151119
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 3
DP  - 2001 Mar
TI  - Expression of the interleukin-18 gene from rhesus macaque by the simian 
      immunodeficiency virus does not result in increased viral replication.
PG  - 173-80
AB  - Interleukin-18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing 
      factor (IGIF), is a proinflammatory cytokine expressed by activated macrophages 
      that acts in synergy with IL-12 as an important amplifying factor for IFN-gamma 
      production and Th1 development. To study the effect of IL-18 on a lentiviral 
      infection, we cloned the IL-18 gene from a rhesus macaque and constructed 
      replication-competent simian immunodeficiency virus (SIV) that expressed either 
      the precursor pro-IL-18 (SIV(IL-18)) or the mature form (SIV(mIL-18)) of IL-18. 
      The predicted amino acid sequence for rhesus IL-18 had 96% homology with the 
      human one, differing in only 8 of 193 residues. SIV(IL-18) and SIV(mIL-18) 
      replicated more slowly than control viruses in the CEM x 174 cell line and 
      resulted in the development of chronically infected cell lines that expressed 
      high levels of infectious SIV. The cell line generated by SIV(IL-18) released 
      large quantities of IL-18 into the supernatant, whereas the one obtained from 
      SIV(mIL-18) showed the accumulation of IL-18 in the cytoplasm. Similarly, 
      SIV(IL-18) and SIV(mIL-18) replicated more slowly than the unmodified viral 
      vector in rhesus peripheral blood mononuclear cells (PMBC), but only SIV(IL-18) 
      expressed biologically active IL-18. These experiments show that the precursor 
      form of IL-18 is necessary for the efficient release of the cytokine and that 
      IL-18 does not promote increased replication of SIV in rhesus PBMC.
FAU - Giavedoni, L D
AU  - Giavedoni LD
AD  - Department of Virology and Immunology, Southwest Foundation for Biomedical 
      Research, San Antonio, TX 78245-0549, USA. Lgiavedo@icarus.sfbr.org
FAU - Imhoof, J D
AU  - Imhoof JD
FAU - Velasquillo, M C
AU  - Velasquillo MC
FAU - Parodi, L M
AU  - Parodi LM
FAU - Hodara, V L
AU  - Hodara VL
LA  - eng
SI  - GENBANK/AF303732
GR  - P51 RR 13986-01/RR/NCRR NIH HHS/United States
GR  - R01 A141923/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Interleukin-18)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/chemistry/genetics/*metabolism
MH  - Leukocytes, Mononuclear/virology
MH  - *Macaca mulatta/genetics/virology
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Simian Immunodeficiency Virus/genetics/*physiology
MH  - *Virus Replication
EDAT- 2001/05/02 10:00
MHDA- 2001/08/03 10:01
CRDT- 2001/05/02 10:00
PHST- 2001/05/02 10:00 [pubmed]
PHST- 2001/08/03 10:01 [medline]
PHST- 2001/05/02 10:00 [entrez]
AID - 10.1089/107999001750133212 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Mar;21(3):173-80. doi: 
      10.1089/107999001750133212.