PMID- 11331945
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20061115
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 12
IP  - 5
DP  - 2001 May
TI  - An integrated cytogenetic, radiation-hybrid, and comparative map of dog 
      chromosome 5.
PG  - 371-5
AB  - The development of a detailed genome map for the domestic dog (Canis familiaris, 
      CFA) is a prerequisite for the continued use of this species as a model system 
      for the study of inherited traits. We present an integrated cytogenetic, 
      radiation-hybrid, and comparative map of dog Chromosome (Chr) 5 (CFA 5). The map 
      comprises 14 gene markers, selected from loci previously mapped within the 
      corresponding evolutionarily conserved chromosome segments (ECCS) of the human 
      genome. Large-insert clones representing each marker were first isolated and 
      mapped by fluorescence in situ hybridization (FISH) analysis to determine their 
      subchromosomal localization on CFA 5. Thirteen gene markers were subsequently 
      mapped by using a commercially available whole genome radiation hybrid (WG-RH) 
      panel for the dog. Nine anonymous markers were also assigned to CFA 5 by both 
      FISH and WG-RH analysis. The 22 markers formed six RH-linkage groups, spanning 
      each of the four ECCS comprising this 99 megabase chromosome. All cytogenetic, 
      WG-RH, and comparative mapping data were in agreement and were combined to 
      determine both the most likely locus order within each linkage group, and also 
      the gross relative orientation of the corresponding ECCS. This study provides a 
      resource for the transfer of information from the human transcript map to that of 
      the dog, and extends existing data regarding the structural relationships between 
      CFA 5 and its evolutionary counterparts within the human genome.
FAU - Thomas, R
AU  - Thomas R
AD  - Genetics Section, Animal Health Trust, Lanwades Park, Kentford, Newmarket, 
      Suffolk, CB8 7UU, UK. rthomas@hgmp.mrc.ac.uk
FAU - Breen, M
AU  - Breen M
FAU - Deloukas, P
AU  - Deloukas P
FAU - Holmes, N G
AU  - Holmes NG
FAU - Binns, M M
AU  - Binns MM
LA  - eng
SI  - GENBANK/AJ293241
SI  - GENBANK/AJ293242
SI  - GENBANK/AJ293243
SI  - GENBANK/AJ293244
SI  - GENBANK/AJ293245
SI  - GENBANK/AJ293246
SI  - GENBANK/AJ293247
SI  - GENBANK/AJ293248
SI  - GENBANK/AJ293249
SI  - GENBANK/AJ293250
SI  - GENBANK/AJ293251
SI  - GENBANK/AJ293252
SI  - GENBANK/AJ293253
SI  - GENBANK/AJ293254
SI  - GENBANK/AJ293255
SI  - GENBANK/AJ293256
SI  - GENBANK/AJ293257
SI  - GENBANK/AJ293258
SI  - GENBANK/AJ293259
SI  - GENBANK/AJ293260
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
SB  - IM
MH  - Animals
MH  - Chromosomes/*genetics
MH  - Dogs
MH  - *In Situ Hybridization, Fluorescence
MH  - Lod Score
MH  - Molecular Sequence Data
MH  - *Radiation Hybrid Mapping
MH  - Sequence Analysis, DNA
EDAT- 2001/05/02 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/05/02 10:00
PHST- 2000/10/17 00:00 [received]
PHST- 2001/01/12 00:00 [accepted]
PHST- 2001/05/02 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/05/02 10:00 [entrez]
AID - 10.1007/s003350010287 [doi]
PST - ppublish
SO  - Mamm Genome. 2001 May;12(5):371-5. doi: 10.1007/s003350010287.