PMID- 11332084
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 23
IP  - 2
DP  - 2001 Apr
TI  - Adjusting interphase FISH results in epithelial tissue sections to whole cell 
      complement.
PG  - 93-100
AB  - OBJECTIVE: To derive an equation to compensate for the discrepancies between 
      whole cell preparations and tissue sections for more accurate enumeration of 
      fluorescence in situ hybridization (FISH) signals per cell. STUDY DESIGN: Mean 
      centromere signal counts in touch preparations and corresponding 4-6-micron 
      sections of paraffin-embedded tissue were calculated. Mean widest nuclear 
      diameters were also determined from the tissue sections. The observed data were 
      analyzed to define the volumetric relationships between tissue sections and whole 
      cell preparations. RESULTS: Analysis of results from six lung specimens yielded 
      an equation that approximates whole versus sectioned nuclear volume and permits 
      accurate quantification of mean FISH signal count in histologic sections, as 
      follows: [formula: see text].
FAU - Berezuk, M
AU  - Berezuk M
AD  - Departments of Pathology and Medicine, University of Colorado Health Sciences 
      Center, 4200 East Ninth Avenue, Campus Box B216, Denver, Colorado 80262, USA.
FAU - West, J
AU  - West J
FAU - Varella-Garcia, M
AU  - Varella-Garcia M
FAU - Franklin, W A
AU  - Franklin WA
LA  - eng
GR  - P50-CA58187/CA/NCI NIH HHS/United States
GR  - U01-CA85070/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Carcinoma/*pathology
MH  - Cell Nucleus/*ultrastructure
MH  - *Cell Size
MH  - DNA, Neoplasm/analysis
MH  - Epithelial Cells/*ultrastructure
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Lung/*cytology
MH  - Lung Neoplasms/*pathology
MH  - Male
MH  - *Mathematics
MH  - Ploidies
EDAT- 2001/05/03 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/05/03 10:00
PHST- 2001/05/03 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/05/03 10:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2001 Apr;23(2):93-100.