PMID- 11333119
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20191025
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 11
IP  - 2
DP  - 2001 Apr
TI  - Detection of invasion-related chromosomal changes in highly and weakly invasive 
      melanoma cell clones by a modified comparative genomic hybridization approach.
PG  - 105-15
AB  - Invasion is a key step in the systemic spread of tumour cells. The aim of this 
      study was to investigate whether specific chromosomal aberrations in melanoma 
      occur during acquisition of a strongly invasive phenotype. We previously selected 
      highly and weakly invasive cell clones from the human melanoma cell line Mel Im. 
      Both cell clones retained a stable phenotype over more than 40 passages, 
      revealing a five-fold difference in their invasive potential in vitro. Direct 
      comparative genomic hybridization (CGH) (modified CGH) of the two cell clones was 
      used as a powerful tool to screen for different chromosomal aberrations in both 
      clones. Standard CGH and fluorescence in situ hybridization (FISH) was performed 
      to verify the results of this improved technique. In general, the CGH pattern 
      showed a high degree of identity consistent with the fact that the cell lines 
      represent subclones of the same cell line. However, a few defined changes between 
      the two cell clones were observed, including loss of 1q21-qter, 4q, 11p14-pter, 
      19q and 20p in the highly invasive cell clone. Two of the regions (1q and 11p) 
      have already been suggested to be involved in melanoma progression, whereas 
      changes in the others have not been detected before. In summary, our direct CGH 
      approach proved to be suitable for fast and direct comparison of two cell types 
      and allowed the identification of two known and three novel chromosomal changes 
      involved in the acquisition of a strongly invasive melanoma cell phenotype.
FAU - Gunther, K
AU  - Gunther K
AD  - University Hospital RWTH Aachen, Institute of Pathology, Germany.
FAU - Fleischer, A
AU  - Fleischer A
FAU - Buettner, R
AU  - Buettner R
FAU - Bosserhoff, A K
AU  - Bosserhoff AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - DNA/metabolism
MH  - Disease Progression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/*genetics/metabolism
MH  - Neoplasm Invasiveness
MH  - *Nucleic Acid Hybridization
MH  - Phenotype
MH  - Tumor Cells, Cultured
EDAT- 2001/05/03 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/05/03 10:00
PHST- 2001/05/03 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/05/03 10:00 [entrez]
AID - 10.1097/00008390-200104000-00004 [doi]
PST - ppublish
SO  - Melanoma Res. 2001 Apr;11(2):105-15. doi: 10.1097/00008390-200104000-00004.