PMID- 11334998
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20190901
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 127
IP  - 2
DP  - 2001 Apr 30
TI  - Parallel induction of the formation of dopamine and its metabolites with 
      induction of tyrosine hydroxylase expression in foetal rat and human cerebral 
      cortical cells by brain-derived neurotrophic factor and glial-cell derived 
      neurotrophic factor.
PG  - 111-22
AB  - Brain-derived neurotrophic factor (BDNF; 50 ng/ml), dopamine (DA; 10 microM) and 
      forskolin (Fsk; 10 microM) have previously been shown by this and other 
      laboratories to induce the tyrosine hydroxylase (TH) enzyme in foetal human and 
      rat cerebral cortex during specified sensitive developmental periods. In the 
      present study, these findings were extended for human and rat cells by showing 
      that the induced TH+ cells also produce dopamine and its metabolite 
      3,4-dihydroxyphenylacetic acid (DOPAC). In addition to this, TH induction and DA 
      plus DOPAC production was observed in foetal human and rat cerebral cortex by 
      using glial-cell derived neurotrophic factor (GDNF) in place of BDNF. The degree 
      of induction by GDNF (1-10 ng/ml) was similar to that produced by BDNF and did 
      not increase further when the two neurotrophic factors were used together. The 
      time-course of induction in human cultures was followed: GDNF was found to cause 
      a more rapid induction process than BDNF during the first 2 weeks. However the 
      degree of induction after 3 weeks was the same for both neurotrophic factors. 
      Inhibitors of transcription (actinomycin D) or of translation (cycloheximide) 
      eliminated all the increase in DA+DOPAC contents elicited by these compounds, 
      indicating that de novo transcription and translation were required for increased 
      expression of the TH and other related enzymes. The intracellular pathways by 
      which these molecules exert this dopaminergic phenotype induction effect are 
      discussed. This study indicates a new source of dopaminergic brain tissue for use 
      as transplants to neurosurgically treat Parkinson's disease patients.
FAU - Theofilopoulos, S
AU  - Theofilopoulos S
AD  - Department of Biochemistry, Imperial College of Science, Technology and Medicine, 
      South Kensington, SW7 2AY, London, UK.
FAU - Goggi, J
AU  - Goggi J
FAU - Riaz, S S
AU  - Riaz SS
FAU - Jauniaux, E
AU  - Jauniaux E
FAU - Stern, G M
AU  - Stern GM
FAU - Bradford, H F
AU  - Bradford HF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GDNF protein, human)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 102-32-9 (3,4-Dihydroxyphenylacetic Acid)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 3,4-Dihydroxyphenylacetic Acid/analysis
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Dactinomycin/pharmacology
MH  - Dopamine/analysis/*biosynthesis/genetics
MH  - Female
MH  - Fetus/cytology/drug effects/enzymology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glutamate Decarboxylase/analysis
MH  - Humans
MH  - Immunohistochemistry
MH  - Microtubule-Associated Proteins/analysis
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/cytology/drug effects/*enzymology
MH  - Neuroprotective Agents/*pharmacology
MH  - Phenotype
MH  - Pregnancy
MH  - Protein Biosynthesis/drug effects
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription, Genetic/drug effects
MH  - Tyrosine 3-Monooxygenase/analysis/genetics/*metabolism
EDAT- 2001/05/04 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/04 10:00
PHST- 2001/05/04 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/04 10:00 [entrez]
AID - S0165380601001250 [pii]
AID - 10.1016/s0165-3806(01)00125-0 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2001 Apr 30;127(2):111-22. doi: 
      10.1016/s0165-3806(01)00125-0.