PMID- 11336444 OWN - NLM STAT- MEDLINE DCOM- 20010913 LR - 20051117 IS - 0897-7151 (Print) IS - 0897-7151 (Linking) VI - 18 IP - 4 DP - 2001 Apr TI - Expression of c-fos, heat shock protein 70, neurotrophins, and cyclooxygenase-2 mRNA in response to focal cerebral ischemia/reperfusion in rats and their modification by magnesium sulfate. PG - 435-45 AB - The marginal area surrounding a region of ischemic brain tissue, designated as the penumbra, is of interest as a potential area for the rescue of neurons from cell death. Despite its clinical importance, relatively little is known about the molecular events leading to changes in brain cells in the penumbra following ischemia. In the first part of this study, we used in situ hybridization to investigate the temporal and spatial expression of c-fos, heat shock protein 70 (HSP70), neurotrophins and inducible cyclooxygenase-2 (COX-2) in the rat brain following a 2-h occlusion of the middle cerebral artery (MCA) with reperfusion. In the penumbra and surrounding cortex, upregulation of c-fos, brain-derived neurotrophic factor (BDNF), and COX-2 mRNAs was observed, while expression of HSP70 mRNA was restricted to the penumbra. This spatial discrepancy of mRNA expression suggests that different mechanisms are involved in the regulation of c-fos/BDNF/COX-2 and HSP70 expression. Intravenous infusion of magnesium sulfate (25 mg/kg) decreased both the infarct volume and upregulation of these mRNAs, suggesting its therapeutic potential. FAU - Kinoshita, Y AU - Kinoshita Y AD - Department of Neurological Surgery, Wakayama Medical University, Wakayama City, Japan. FAU - Ueyama, T AU - Ueyama T FAU - Senba, E AU - Senba E FAU - Terada, T AU - Terada T FAU - Nakai, K AU - Nakai K FAU - Itakura, T AU - Itakura T LA - eng PT - Journal Article PL - United States TA - J Neurotrauma JT - Journal of neurotrauma JID - 8811626 RN - 0 (Isoenzymes) RN - 0 (Nerve Growth Factors) RN - 0 (Proto-Oncogene Proteins c-fos) RN - 0 (RNA, Messenger) RN - 7487-88-9 (Magnesium Sulfate) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases) SB - IM MH - Animals MH - Brain/metabolism/pathology MH - Brain Ischemia/*metabolism MH - Cerebral Infarction/pathology MH - Cyclooxygenase 2 MH - Isoenzymes/*genetics MH - Magnesium Sulfate/*pharmacology MH - Male MH - Nerve Growth Factors/*genetics MH - Prostaglandin-Endoperoxide Synthases/*genetics MH - Proto-Oncogene Proteins c-fos/*genetics MH - RNA, Messenger/*metabolism MH - Rats MH - Reperfusion Injury/*metabolism MH - Time Factors MH - Up-Regulation EDAT- 2001/05/05 10:00 MHDA- 2001/09/14 10:01 CRDT- 2001/05/05 10:00 PHST- 2001/05/05 10:00 [pubmed] PHST- 2001/09/14 10:01 [medline] PHST- 2001/05/05 10:00 [entrez] AID - 10.1089/089771501750171038 [doi] PST - ppublish SO - J Neurotrauma. 2001 Apr;18(4):435-45. doi: 10.1089/089771501750171038.