PMID- 11339836
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20231213
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 266
IP  - 1
DP  - 2001 May 15
TI  - A new alternatively spliced transcript of the mouse connexin32 gene is expressed 
      in embryonic stem cells, oocytes, and liver.
PG  - 177-86
AB  - The rodent gap junction protein connexin32 (Cx32) is highly expressed in 
      hepatocytes, less abundantly in Schwann cells and oligodendrocytes, and at low 
      levels in the early mouse embryo. In both hepatocytes and Schwann cells, Cx32 
      expression is directed by alternative promoter regions (P1 and P2) which activate 
      differently spliced transcript isoforms. Here we describe a third Cx32 transcript 
      isoform expressed in embryonic cells, oocytes, and liver. Using competitive 
      polymerase chain reaction, we have found that this new Cx32 transcript containing 
      exon 1A is 200-fold less abundant in liver than the Cx32 isoform with exon 1. In 
      mouse oocytes, the exon 1A-containing Cx32 transcript is exclusively expressed. 
      Immunoblot analyses revealed no Cx32 protein expression in embryonic stem cells, 
      whereas it has previously been demonstrated in oocytes. When the putative Cx32 
      promoter region upstream of exon 1A was cloned before the lacZ reporter gene, 
      transient transfection yielded weak expression in embryonic stem cells. Our 
      results suggest that the exon 1A-containing Cx32 isoform is likely to be 
      inherited as an oogenetic product but not translated during early embryogenesis.
CI  - Copyright 2001 Academic Press.
FAU - Sohl, G
AU  - Sohl G
AD  - Institut fur Genetik, Albeilung Molekulargenetik, Universitat Bonn, 53117 Bonn, 
      Germany.
FAU - Theis, M
AU  - Theis M
FAU - Hallas, G
AU  - Hallas G
FAU - Brambach, S
AU  - Brambach S
FAU - Dahl, E
AU  - Dahl E
FAU - Kidder, G
AU  - Kidder G
FAU - Willecke, K
AU  - Willecke K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Connexins)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Alternative Splicing/*physiology
MH  - Animals
MH  - Cells, Cultured/cytology/metabolism
MH  - Connexins/*genetics/*metabolism
MH  - Embryo, Mammalian/cytology/*embryology/metabolism
MH  - Exons/physiology
MH  - Gene Expression Regulation, Developmental/physiology
MH  - Genes/genetics
MH  - Liver/cytology/*metabolism
MH  - Mice
MH  - Oocytes/cytology/*metabolism
MH  - Promoter Regions, Genetic/physiology
MH  - Protein Isoforms/genetics/metabolism
MH  - Stem Cells/cytology/*metabolism
MH  - Gap Junction beta-1 Protein
EDAT- 2001/05/08 10:00
MHDA- 2001/06/15 10:01
CRDT- 2001/05/08 10:00
PHST- 2001/05/08 10:00 [pubmed]
PHST- 2001/06/15 10:01 [medline]
PHST- 2001/05/08 10:00 [entrez]
AID - S0014-4827(01)95209-7 [pii]
AID - 10.1006/excr.2001.5209 [doi]
PST - ppublish
SO  - Exp Cell Res. 2001 May 15;266(1):177-86. doi: 10.1006/excr.2001.5209.