PMID- 11340305
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20191210
IS  - 1018-7782 (Print)
IS  - 1018-7782 (Linking)
VI  - 9
IP  - 3
DP  - 2001
TI  - Regulation of tubular cell MCP-1 production by intracellular ions: a role for 
      sodium and calcium.
PG  - 205-13
AB  - Proximal tubule cells (PTC) in chronic renal disease produce chemokines which 
      cause renal interstitial inflammation, and also transport more Na+ than normal. 
      To investigate whether these two events might be related, monocyte 
      chemoattractant protein-1 (MCP-1) production was examined in rat PTC in primary 
      culture. Amiloride reduced, while ouabain increased levels of MCP-1 mRNA and 
      protein. Amiloride reduced MCP-1 in cells stimulated with ouabain, 
      lipopolysaccharide (LPS) or albumin. Intracellular Na+ rose with ouabain, but not 
      LPS or albumin. Effects of amiloride, ouabain, LPS and albumin were abrogated by 
      sodium-free but not chloride-free culture medium, and were not explained by 
      changes in intracellular pH. Intracellular Ca2+ rose with ouabain, LPS or albumin 
      and sodium-free medium. BAPTA-AM reduced intracellular Ca2+ and MCP-1 mRNA levels 
      in unstimulated cells, and cells stimulated with ouabain, LPS or albumin. Thus, 
      amiloride and ouabain may alter tubular cell MCP-1 by changing intracellular Na+, 
      with secondary changes in intracellular Ca2+, whereas stimulation by LPS and 
      albumin may involve Ca2+ directly.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Harris, D C
AU  - Harris DC
AD  - Department of Renal Medicine, The University of Sydney at Westmead Hospital, 
      Westmead, NSW, Australia. dch@medicine.usyd.edu.au
FAU - Wang, Y
AU  - Wang Y
FAU - Campbell, D
AU  - Campbell D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Nephrol
JT  - Experimental nephrology
JID - 9302239
RN  - 0 (Chemokine CCL2)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 139890-68-9 (1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid 
      acetoxymethyl ester)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 5ACL011P69 (Ouabain)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - SY7Q814VUP (Calcium)
RN  - VW50CE070T (ethylisopropylamiloride)
SB  - IM
MH  - Acid-Base Equilibrium/physiology
MH  - Amiloride/analogs & derivatives/*pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors/biosynthesis/physiology
MH  - Dose-Response Relationship, Drug
MH  - Egtazic Acid/analogs & derivatives/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Dyes
MH  - Hydrogen-Ion Concentration
MH  - Kidney Tubules, Proximal/*drug effects/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Ouabain/*pharmacology
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium/*metabolism
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
MH  - Spectrometry, Fluorescence
EDAT- 2001/05/08 10:00
MHDA- 2001/08/17 10:01
CRDT- 2001/05/08 10:00
PHST- 2001/05/08 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/05/08 10:00 [entrez]
AID - 52613 [pii]
AID - 10.1159/000052613 [doi]
PST - ppublish
SO  - Exp Nephrol. 2001;9(3):205-13. doi: 10.1159/000052613.