PMID- 11340674
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20061115
IS  - 1059-910X (Print)
IS  - 1059-910X (Linking)
VI  - 53
IP  - 4
DP  - 2001 May 15
TI  - Role of monocyte chemoattractant protein-1 in Propionibacterium acnes-induced 
      pulmonary granulomatosis.
PG  - 288-97
AB  - The inflammatory process in granulomatous disorders such as sarcoidosis is mainly 
      the consequence of delayed hypersensitivity induced by causative antigens. 
      Propionibacterial DNA was isolated recently by PCR from human sarcoidosis tissue. 
      Hence, we developed a model using sensitized rabbits for T cell-mediated 
      pulmonary granulomatosis induced by Propionibacterium acnes (P. acnes) and 
      investigated the role of monocyte chemoattractant protein-1 (MCP-1) in the 
      pathogenesis of the granuloma formation in vivo. Intravenous injection of P. 
      acnes into sensitized rabbits induced massive pulmonary granulomas on day 3. 
      Maximum levels of MCP-1 in sera and bronchoalveolar lavage fluid (BALF) were 
      detected on day 1 and preceded recruitment of monocyte/macrophages and T cells. 
      In BALF, monocyte chemotaxis peaked 1 day after P. acnes challenge, and T cell 
      chemotaxis peaked 3 days after P. acnes challenge. Anti-MCP-1 IgG inhibited 
      monocyte chemotaxis by 80.2% and T cell chemotaxis by 35.7%. Phenotypic analysis 
      of migrating T cells revealed that activated and memory T cells 
      (CD26(+)/CD45RO(+)) but not naive cells were preferentially attracted to BALF. 
      Administration of MCP-1 antiserum in vivo inhibited the development of granulomas 
      in both size 59.9% reduction and number 28.6% reduction, the number of 
      infiltrating leukocytes in BALF, and the expression of adhesion molecules on 
      leukocytes in peripheral blood and BALF. Our data indicate that MCP-1 plays 
      important roles in granuloma formation by attracting and activating specific 
      types of cells in this model. Furthermore, results suggest that the rabbit model 
      resembles human angiocentric granulomatosis and would be useful for investigating 
      the immunopathogenesis of human pulmonary granulomatosis.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Ichiyasu, H
AU  - Ichiyasu H
AD  - First Department of Internal Medicine, Kumamoto University School of Medicine, 
      Kumamoto, Japan.
FAU - Suga, M
AU  - Suga M
FAU - Iyonaga, K
AU  - Iyonaga K
FAU - Ando, M
AU  - Ando M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*immunology
MH  - Disease Models, Animal
MH  - Gram-Positive Bacterial Infections/*immunology/microbiology
MH  - Granuloma, Respiratory Tract/*immunology/microbiology
MH  - Humans
MH  - Immune Sera
MH  - Lung Diseases/*immunology/microbiology
MH  - Propionibacterium acnes/*immunology/pathogenicity
MH  - Rabbits
MH  - T-Lymphocytes/immunology/pathology
EDAT- 2001/05/08 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/05/08 10:00
PHST- 2001/05/08 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/05/08 10:00 [entrez]
AID - 10.1002/jemt.1095 [pii]
AID - 10.1002/jemt.1095 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2001 May 15;53(4):288-97. doi: 10.1002/jemt.1095.