PMID- 11341968
OWN - NLM
STAT- MEDLINE
DCOM- 20010521
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1499
IP  - 3
DP  - 2001 Jan 15
TI  - Regulation of phospholipase D (PLD) in growth plate chondrocytes by 
      24R,25-(OH)2D3 is dependent on cell maturation state (resting zone cells) and is 
      specific to the PLD2 isoform.
PG  - 209-21
AB  - Many of the effects of 1alpha,25-(OH)2D3 and 24R,25-(OH)2D3 on costochondral 
      chondrocytes are mediated by the protein kinase C (PKC) signal transduction 
      pathway. 1alpha,25-(OH)2D3 activates PKC in costochondral growth zone 
      chondrocytes through a specific membrane receptor (1alpha,25-mVDR), involving 
      rapid increases in diacylglycerol via a phospholipase C (PLC)-dependent 
      mechanism. 24R,25-(OH)2D3 activates PKC in resting zone chondrocytes. Although 
      diacylglycerol is increased by 24R,25-(OH)2D3, PLC is not involved, suggesting a 
      phospholipase D (PLD)-dependent mechanism. Here, we show that resting zone and 
      growth zone cells express mRNAs for PLD1a, PLD1b, and PLD2. Both cell types have 
      PLD activity, but levels are higher in resting zone cells. 24R,25-(OH)2D3, but 
      not 24S,25-(OH)2D3 or 1alpha,25-(OH)2D3, stimulates PLD activity in resting zone 
      cells within 3 min via nongenomic mechanisms. Neither 1alpha,25-(OH)2D3 nor 
      24R,25-(OH)2D3 affected PLD in growth zone cells. Basal and 
      24R,25-(OH)2D3-stimulated PLD were inhibited by the PLD inhibitors wortmannin and 
      EDS. Inhibition of phosphatidylinositol 3-kinase (PI 3-kinase), PKC, 
      phosphatidylinositol-specific PLC (PI-PLC), and phosphatidylcholine-specific PLC 
      (PC-PLC) had no effect on PLD activity. Thus, 24R,25-(OH)2D3 stimulates PLD, and 
      PI 3-kinase, PI-PLC and PKC are not involved, whereas PLD is required for 
      stimulation of PKC by 24R,25-(OH)2D3. Pertussis toxin, GDPbetaS, and GTPgammaS 
      had no effect on 24R,25-(OH)2D3-dependent PLD when added to cell cultures, 
      indicating that G-proteins are not involved. These data show that PKC activation 
      in resting zone cells is mediated by PLD and suggest that a functional 
      24R,25-(OH)2D3-mVDR is required. The results also support the conclusion that the 
      24R,25-(OH)2D3-responsive PLD is PLD2, since this PLD isoform is 
      G-protein-independent.
FAU - Sylvia, V L
AU  - Sylvia VL
AD  - Department of Orthopaedics, University of Texas Health Science Center at San 
      Antonio, TX 78229-3900, USA.
FAU - Schwartz, Z
AU  - Schwartz Z
FAU - Del Toro, F
AU  - Del Toro F
FAU - DeVeau, P
AU  - DeVeau P
FAU - Whetstone, R
AU  - Whetstone R
FAU - Hardin, R R
AU  - Hardin RR
FAU - Dean, D D
AU  - Dean DD
FAU - Boyan, B D
AU  - Boyan BD
LA  - eng
GR  - DE-05937/DE/NIDCR NIH HHS/United States
GR  - DE-08603/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Androstadienes)
RN  - 0 (DNA Primers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 40013-87-4 (24,25-Dihydroxyvitamin D 3)
RN  - EC 3.1.4.- (phospholipase D2)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - NGZ37HRE42 (Sphingosine)
RN  - OWA98U788S (safingol)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - 24,25-Dihydroxyvitamin D 3/*pharmacology
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/*drug effects/*enzymology
MH  - DNA Primers/genetics
MH  - Enzyme Inhibitors/pharmacology
MH  - GTP-Binding Proteins/metabolism
MH  - Growth Plate/cytology/*drug effects/*enzymology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Phospholipase D/antagonists & inhibitors/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Sphingosine/*analogs & derivatives/pharmacology
MH  - Wortmannin
EDAT- 2001/05/09 10:00
MHDA- 2001/05/25 10:01
CRDT- 2001/05/09 10:00
PHST- 2001/05/09 10:00 [pubmed]
PHST- 2001/05/25 10:01 [medline]
PHST- 2001/05/09 10:00 [entrez]
AID - S0167-4889(00)00120-8 [pii]
AID - 10.1016/s0167-4889(00)00120-8 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2001 Jan 15;1499(3):209-21. doi: 
      10.1016/s0167-4889(00)00120-8.