PMID- 11341971
OWN - NLM
STAT- MEDLINE
DCOM- 20010521
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1499
IP  - 3
DP  - 2001 Jan 15
TI  - Growth condition-dependent synchronized changes in transcript levels of type II 
      hexokinase and type 1 glucose transporter in tumor cells.
PG  - 242-8
AB  - Transcript levels of hexokinase (HK) isozymes and glucose transporter (GLUT) 
      isoforms in RNA samples of AH130 cells obtained from dish cultures and ascites 
      were evaluated in a quantitative manner. In AH130 cells cultured in dishes, HKI 
      and HKII were expressed at a similar level, but HKIII and HKIV were not. GLUT1 
      and GLUT3 were also expressed, and messages of these two isoforms represented 27% 
      and 71%, respectively, of the total GLUT messages. A faint signal of GLUT2 was 
      also observed. On the contrary, in cells grown as ascites, the transcript of HKII 
      was dominant, and its level was about 15-fold over that of dish-cultured AH130 
      cells. Transcript levels of GLUT1 and GLUT3 were 4.5- and 2-fold, respectively, 
      higher than those in dish-cultured cells. Thus, GLUT1 was more susceptible to 
      changes in culture conditions than GLUT3. Based on these results, we concluded 
      that the change in growth conditions caused synchronized changes in the 
      transcript levels of HKII and GLUT1 in AH130 cells. However, such marked changes 
      in the transcript levels of HKII and GLUT1 were not observed when AH130 cells 
      were cultured in dishes under a hypoxic condition, indicating that the observed 
      changes were not solely attributable to the difference in oxygen concentration 
      between the ascites and cell culture conditions. Accordingly, other factors such 
      as growth factors may be responsible for this difference in levels of HKII and 
      GLUT1 between the two growth conditions.
FAU - Shinohara, Y
AU  - Shinohara Y
AD  - Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi-1, 
      770-8505, Tokushima, Japan. yasuo@ph.tokushima-u.ac.jp
FAU - Hino, M
AU  - Hino M
FAU - Ishida, T
AU  - Ishida T
FAU - Yamanaka, Y
AU  - Yamanaka Y
FAU - Terada, H
AU  - Terada H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA Primers)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Isoenzymes)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Slc2a1 protein, rat)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Division
MH  - Cell Hypoxia/genetics/physiology
MH  - DNA Primers/genetics
MH  - Glucose Transporter Type 1
MH  - Hexokinase/*genetics
MH  - Isoenzymes/genetics
MH  - Liver Neoplasms, Experimental/genetics/metabolism/pathology
MH  - Male
MH  - Monosaccharide Transport Proteins/*genetics
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/*genetics/*metabolism
MH  - RNA, Neoplasm/*genetics/*metabolism
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 2001/05/09 10:00
MHDA- 2001/05/25 10:01
CRDT- 2001/05/09 10:00
PHST- 2001/05/09 10:00 [pubmed]
PHST- 2001/05/25 10:01 [medline]
PHST- 2001/05/09 10:00 [entrez]
AID - S0167-4889(00)00125-7 [pii]
AID - 10.1016/s0167-4889(00)00125-7 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2001 Jan 15;1499(3):242-8. doi: 
      10.1016/s0167-4889(00)00125-7.