PMID- 11343210
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20131121
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 183
IP  - 11
DP  - 2001 Jun 1
TI  - In vivo expression of proinflammatory mediators in the adult heart after 
      endotoxin administration: the role of toll-like receptor-4.
PG  - 1617-24
AB  - Tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and nitric oxide (NO) 
      may play a role in lipopolysaccharide (LPS)-induced cardiac depression. Toll-like 
      receptor-4 (TLR-4) mediates the cytokine response to LPS in immune cells. TLR-4 
      also is expressed in human and murine myocardial tissue. Therefore, the 
      hypothesis that LPS induces proinflammatory cytokines in the heart via TLR-4 was 
      tested. C3H/HeJ (TLR-4 deficient) and C3HeB/FeJ mice were studied. LPS induced a 
      robust increase in myocardial TNF-alpha and IL-1beta mRNA in C3HeB/FeJ mice. The 
      response in C3H/HeJ mice was blunted and delayed. Myocardial TNF-alpha and 
      IL-1beta protein levels were higher in C3HeB/FeJ mice, as were inducible NO 
      synthase protein and NO production. Activation of myocardial NF-kappaB was 
      observed within 30 min in C3HeB/FeJ mice but not in C3H/HeJ mice. These findings 
      suggest that myocardial TLR-4 is involved in signaling cytokine production within 
      the heart during endotoxic shock.
FAU - Baumgarten, G
AU  - Baumgarten G
AD  - Department of Medicine, Cardiology Section, Winters Center for Heart Failure 
      Research, and Veterans Affairs Medical Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Knuefermann, P
AU  - Knuefermann P
FAU - Nozaki, N
AU  - Nozaki N
FAU - Sivasubramanian, N
AU  - Sivasubramanian N
FAU - Mann, D L
AU  - Mann DL
FAU - Vallejo, J G
AU  - Vallejo JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010423
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Drosophila Proteins)
RN  - 0 (Endotoxins)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - *Drosophila Proteins
MH  - Endotoxins/*pharmacology
MH  - Heart/*drug effects
MH  - Interleukin-1/metabolism
MH  - Lipopolysaccharides
MH  - Membrane Glycoproteins/deficiency/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Knockout
MH  - Myocardium/*metabolism
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Receptors, Cell Surface/deficiency/genetics/*physiology
MH  - Signal Transduction
MH  - Time Factors
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2001/05/09 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/05/09 10:00
PHST- 2000/12/11 00:00 [received]
PHST- 2001/02/26 00:00 [revised]
PHST- 2001/05/09 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/05/09 10:00 [entrez]
AID - JID001477 [pii]
AID - 10.1086/320712 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Jun 1;183(11):1617-24. doi: 10.1086/320712. Epub 2001 Apr 23.