PMID- 11343771
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20220409
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 126
IP  - 1
DP  - 2001 Apr 1
TI  - Aberrations of chromosome 8 in 16 breast cancer cell lines by comparative genomic 
      hybridization, fluorescence in situ hybridization, and spectral karyotyping.
PG  - 1-7
AB  - Comparative genomic hybridization (CGH) studies have shown that chromosome 8 is a 
      frequent target for chromosomal aberrations in breast cancer. We characterized 
      these aberrations of chromosome 8 in 16 breast cancer cell lines (BT-474, BT-549, 
      CAMA-1, DU-4475, MCF-7, MDA-MB-134, MDA-MB-157, MDA-MB-361, MDA-MB-415, 
      MDA-MB-436, MPE600, SK-BR-3, T-47D, UACC-812, UACC-893 and ZR-75-1) by CGH, 
      fluorescence in situ hybridization (FISH) with arm- and locus-specific probes, 
      and spectral karyotyping (SKY). Chromosome 8 was structurally abnormal in 13 of 
      16 cell lines. Loss of 8p was detected in nine cell lines, gain of entire 8q in 
      six cell lines, 8q21-qter in three, 8q23-qter in two, and 8q12-qter and 8p21-q21 
      in one cell line. Extra copies of the C-MYC oncogene were found in 11 cell lines, 
      but high-level amplification only in SK-BR-3. Derivative chromosomes including 
      material from chromosomes 8 were complex, and the breakpoints were strikingly 
      dissimilar. Chromosome 11 was the most frequent translocation partner with 
      chromosome 8 (in 7 cell lines). Isochromosomes and/or isoderivative 8q were found 
      in four cell lines. The high frequency and complexity of alterations at 8q 
      indicate a significant pathogenetic role in breast cancer. The high-level 
      amplification of c-myc is less common than previously thought.
FAU - Rummukainen, J
AU  - Rummukainen J
AD  - Laboratory of Cancer Genetics, Institute of Medical Technology, University of 
      Tampere and Tampere University Hospital, FIN-33101, Tampere, Finland.
FAU - Kytola, S
AU  - Kytola S
FAU - Karhu, R
AU  - Karhu R
FAU - Farnebo, F
AU  - Farnebo F
FAU - Larsson, C
AU  - Larsson C
FAU - Isola, J J
AU  - Isola JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - Karyotyping/methods
MH  - Nucleic Acid Hybridization/methods
MH  - Tumor Cells, Cultured
EDAT- 2001/05/10 10:00
MHDA- 2001/06/15 10:01
CRDT- 2001/05/10 10:00
PHST- 2001/05/10 10:00 [pubmed]
PHST- 2001/06/15 10:01 [medline]
PHST- 2001/05/10 10:00 [entrez]
AID - S0165-4608(00)00387-3 [pii]
AID - 10.1016/s0165-4608(00)00387-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Apr 1;126(1):1-7. doi: 
      10.1016/s0165-4608(00)00387-3.