PMID- 11343855
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190901
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 118
IP  - 1
DP  - 2001 Apr 15
TI  - The expression of excitatory amino acid transporter 2 (EAAT2) in forensic autopsy 
      cases.
PG  - 49-55
AB  - Glutamate is the major excitatory neurotransmitter and the greater part of this 
      amino acid is removed from the synaptic cleft by excitatory amino acid 
      transporter 2 (EAAT2) located on perisynaptic astrocytes. Recently, it was 
      reported that the EAAT2 protein content changed in rats following forebrain 
      ischemia and administration of methamphetamine. We planned to demonstrate the 
      immunohistochemical distribution of EAAT2 in the human brain and discuss the 
      significance of its pathophysiological roles. Thirty-two cases were used from 
      forensic autopsies. The tissues were sampled from the cerebral cortex, striatum 
      and hippocampus. The distribution of EAAT2 was difficult to identify in cases of 
      electrical fatalities. However, continuous and extensive staining of EAAT2 was 
      observed in cases of death from hypothermia. In almost all asphyxia death, we 
      were able to observe a weak stain of EAAT2. In case of solvent abuse, EAAT2 
      staining was continuous and extensive as in the cases of hypothermia, and patchy 
      negative zones were mixed. This study clearly showed the differences in EAAT2 
      localization according to the cause of death. These findings suggested that the 
      differences in EAAT2 staining depended on the cause and course 
      (pathophysiological conditions) of death.
FAU - Ikematsu, K
AU  - Ikematsu K
AD  - Department of Legal Medicine, Nagasaki University School of Medicine, 1-12-4 
      Sakamoto, 852-8523, Nagasaki, Japan.
FAU - Tsuda, R
AU  - Tsuda R
FAU - Orihara, Y
AU  - Orihara Y
FAU - Nakasono, I
AU  - Nakasono I
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Receptors, Neurotransmitter)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Autopsy
MH  - Brain/*metabolism/pathology
MH  - *Cause of Death
MH  - Child
MH  - Excitatory Amino Acid Transporter 2
MH  - Female
MH  - Forensic Medicine/*methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Receptors, Neurotransmitter/*metabolism
MH  - Time Factors
EDAT- 2001/05/10 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/05/10 10:00
PHST- 2001/05/10 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/05/10 10:00 [entrez]
AID - S0379073800003789 [pii]
AID - 10.1016/s0379-0738(00)00378-9 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2001 Apr 15;118(1):49-55. doi: 10.1016/s0379-0738(00)00378-9.