PMID- 11344508
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20220408
IS  - 0196-8092 (Print)
IS  - 0196-8092 (Linking)
VI  - 28
IP  - 4
DP  - 2001
TI  - Laser-tissue soldering with biodegradable polymer films in vitro: film surface 
      morphology and hydration effects.
PG  - 297-306
AB  - BACKGROUND AND OBJECTIVE: Previous research introduced the concept of using 
      biodegradable polymer film reinforcement of a liquid albumin solder for 
      improvement of the tensile strength of repaired incisions in vitro. In this 
      study, the effect of creating small pores in the PLGA films on the weld breaking 
      strength is studied. Additionally, the effect of hydration on the strength of the 
      reinforced welds is investigated. STUDY DESIGN/MATERIALS AND METHODS: A 50%(w/v) 
      bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to 
      repair an incision in bovine aorta. The solder was coagulated with an 806-nm CW 
      diode laser. A poly(DL-lactic-co-glycolic acid) (PLGA) film was used to reinforce 
      the solder (the controls had solder but no reinforcement). Breaking strengths 
      were measured acutely and after hydration in saline for 1 and 2 days. The data 
      were analyzed by ANOVA (P < 0.05) and multiple comparisons of means were 
      performed using the Newman-Keuls test. RESULTS: The creation of pores in the PLGA 
      films qualitatively improved the film flexibility without having an apparent 
      adverse effect on the breaking strength, while the actual technique of applying 
      the film and solder had more of an effect. The acute maximum average breaking 
      strengths of some of the film reinforced specimens (114.7 g-134.4 g) were 
      significantly higher (P < 0.05) than the acute maximum average breaking strength 
      of the unreinforced control specimens (68.3 g). Film reinforced specimens were 
      shown to have a statistically significantly higher breaking strength than 
      unreinforced controls after 1- and 2-day hydration. CONCLUSIONS: Reinforcement of 
      liquid albumin solders in laser-assisted incision repair appears to have 
      advantages over conventional methods that do not reinforce the cohesive strength 
      of the solder in terms of acute breaking strength and after immersion in moist 
      environments for short periods of time. Using a film with the solder applied to 
      one surface only may be advantageous over other techniques.
FAU - Sorg, B S
AU  - Sorg BS
AD  - Biomedical Engineering Program, University of Texas at Austin, Austin, Texas 
      78712, USA. sorg@mail.utexas.edu
FAU - Welch, A J
AU  - Welch AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lasers Surg Med
JT  - Lasers in surgery and medicine
JID - 8007168
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/surgery
MH  - Biocompatible Materials
MH  - Biodegradation, Environmental
MH  - Cattle
MH  - In Vitro Techniques
MH  - *Lactic Acid
MH  - *Laser Coagulation
MH  - Microscopy, Electron, Scanning
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Prostheses and Implants
MH  - *Serum Albumin, Bovine
MH  - Tensile Strength
EDAT- 2001/05/10 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/05/10 10:00
PHST- 2001/05/10 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/05/10 10:00 [entrez]
AID - 10.1002/lsm.1053 [doi]
PST - ppublish
SO  - Lasers Surg Med. 2001;28(4):297-306. doi: 10.1002/lsm.1053.