PMID- 11346892
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20191104
IS  - 0379-0355 (Print)
IS  - 0379-0355 (Linking)
VI  - 22
IP  - 10
DP  - 2000 Dec
TI  - Evidence that Toki-shakuyaku-san and its ingredients enhance the secretion of a 
      cytokine-induced neutrophil chemoattractant (CINC/gro) in the ovulatory process.
PG  - 725-30
AB  - We investigated the effects of Toki-shakuyaku-san and its crude ingredients in 
      relation to the secretion of a cytokine-induced neutrophil chemoattractant, 
      CINC/gro, interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF 
      alpha) in the ovulatory process. Toki-shakuyaku-san significantly (p < 0.01) 
      stimulated the secretion of 17 beta-estradiol but did not stimulate the secretion 
      of progesterone in cultured whole ovarian dispersates. Toki-shakuyaku-san 
      enhanced the secretion of CINC/gro in a dose-dependent manner and the production 
      of CINC/gro at concentrations of 10 and 100 micrograms/ml of Toki-shakuyaku-san 
      increased significantly (p < 0.01). Toki-shakuyaku-san also enhanced secretions 
      of both IL-1 beta and TNF alpha, which are known to stimulate the secretion of 
      CINC/gro in the ovulatory process. The production of TNF alpha increased 
      significantly (p < 0.05) with 10 and 100 micrograms/ml of Toki-shakuyaku-san. 
      Atractylodis Lanceae Rhizoma, Cnidii Rhizoma, Angelicae Radix, Paeoniae Radix and 
      Alismatis Rhizoma, which are crude ingredients of Toki-shakuyaku-san, 
      significantly (p < 0.01) enhanced the secretion of CINC/gro at concentrations of 
      100 micrograms/ml. The results of this study show that Toki-shakuyaku-san can 
      stimulate the secretion of 17 beta-estradiol and stimulate the ovulatory process 
      by stimulating the production of CINC/gro, IL-1 beta and TNF alpha in vitro. As a 
      treatment for ovulatory disorders, Toki-shakuyaku-san may have stimulatory 
      effects on both steroidogenesis and the ovulatory process.
FAU - Irahara, M
AU  - Irahara M
AD  - Department of Obstetrics and Gynecology, School of Medicine, University of 
      Tokushima, Tokushima, Japan.
FAU - Yasui, T
AU  - Yasui T
FAU - Tezuka, M
AU  - Tezuka M
FAU - Ushigoe, K
AU  - Ushigoe K
FAU - Yamano, S
AU  - Yamano S
FAU - Kamada, M
AU  - Kamada M
FAU - Aono, T
AU  - Aono T
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Methods Find Exp Clin Pharmacol
JT  - Methods and findings in experimental and clinical pharmacology
JID - 7909595
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Cxcl1 protein, rat)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (toki-shakuyaku-san)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CXCL1
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*metabolism
MH  - Culture Media, Serum-Free
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Estradiol/metabolism
MH  - Female
MH  - Growth Substances/*metabolism
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Interleukin-1/metabolism
MH  - Ovary/cytology/*drug effects/metabolism
MH  - Ovulation/*physiology
MH  - Plants, Medicinal/chemistry
MH  - Progesterone/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2001/05/12 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/05/12 10:00
PHST- 2001/05/12 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/05/12 10:00 [entrez]
AID - 802288 [pii]
AID - 10.1358/mf.2000.22.10.802288 [doi]
PST - ppublish
SO  - Methods Find Exp Clin Pharmacol. 2000 Dec;22(10):725-30. doi: 
      10.1358/mf.2000.22.10.802288.