PMID- 11350638
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20191104
IS  - 1067-1927 (Print)
IS  - 1067-1927 (Linking)
VI  - 9
IP  - 1
DP  - 2001 Jan-Feb
TI  - Phenotypic variation in the production of bioactive hepatocyte growth 
      factor/scatter factor by oral mucosal and skin fibroblasts.
PG  - 34-43
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor 
      produced principally by cells of mesenchymal origin. HGF/SF is an important 
      mitogen, morphogen, and motogen and plays an important role in wound healing, 
      tumorigenesis and particularly fetal development. Oral mucosal fibroblasts 
      exhibit a fetal phenotype, including an increased extracellular matrix 
      reorganizational ability, cellular migration and experimental wound repopulation 
      in comparison to skin fibroblasts. In this study the expression, production, and 
      bioactivity of HGF/SF by oral mucosal and skin fibroblasts was investigated. 
      Although both oral mucosal and skin fibroblasts expressed HGF/SF, the oral 
      mucosal fibroblasts produced significantly increased amounts of total HGF/SF (p < 
      0.01) as measured by enzyme-linked immunosorbent assay and bioactive HGF/SF as 
      measured by cell scatter and cell-dissociation techniques (p < 0.01). The 
      possible effect of increased HGF/SF in production mediating the previously 
      described preferential responses of oral mucosal fibroblasts was studied in 
      vitro. Reverse transcriptase-polymerase chain reaction-Western blotting and 
      immunocytochemistry methods all showed that both oral mucosal and skin 
      fibroblasts expressed and produced the c-Met receptor. Recombinant HGF (20-40 
      ng/mL) however, failed to affect fibroblast repopulation of monolayer wounds or 
      cellular proliferation. In contrast, recombinant HGF significantly increased 
      ECV304 wound repopulation. These studies provide direct evidence of another 
      mechanism by which site-specific variations in fibroblast phenotype may 
      contribute in a paracrine fashion to the rapid reepithelialization and 
      revascularization of oral wounds.
FAU - Stephens, P
AU  - Stephens P
AD  - Department of Oral Surgery, Medicine and Pathology, University of Wales College 
      of Medicine, Cardiff, United Kingdom.
FAU - Hiscox, S
AU  - Hiscox S
FAU - Cook, H
AU  - Cook H
FAU - Jiang, W G
AU  - Jiang WG
FAU - Zhiquiang, W
AU  - Zhiquiang W
FAU - Thomas, D W
AU  - Thomas DW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Wound Repair Regen
JT  - Wound repair and regeneration : official publication of the Wound Healing Society 
      [and] the European Tissue Repair Society
JID - 9310939
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adolescent
MH  - Blotting, Western
MH  - Child
MH  - Child, Preschool
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fibroblasts/*physiology
MH  - Hepatocyte Growth Factor/analysis/*metabolism/pharmacology
MH  - Humans
MH  - Infant
MH  - Male
MH  - Mouth Mucosa/*physiology
MH  - Phenotype
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Skin/cytology
MH  - Wound Healing
EDAT- 2001/05/15 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/05/15 10:00
PHST- 2001/05/15 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/05/15 10:00 [entrez]
AID - wrr034 [pii]
AID - 10.1046/j.1524-475x.2001.00034.x [doi]
PST - ppublish
SO  - Wound Repair Regen. 2001 Jan-Feb;9(1):34-43. doi: 
      10.1046/j.1524-475x.2001.00034.x.