PMID- 11350690
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20061115
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 9
IP  - 2
DP  - 2001 Apr
TI  - [Recombinant human growth hormone downregulates the apoptosis of HepG(2) cells 
      induced by LPS].
PG  - 100-2
AB  - OBJECTIVE: To investigate the effect of LPS on human hepatocytes and study 
      whether recombinant human growth hormone (rhGH) could protect hepatocytes from 
      apoptosis induced by LPS. METHODS: HepG(2) cells were treated with LPS (20 
      microg/ml) or LPS and rhGH for 16 hours. The apoptosis of HepG(2) cells was 
      detected by terminal deoxynucleotide transferase-mediated dUTP nick end labeling 
      (TUNEL) or electron microscopy. RESULTS: HepG(2) cells treated with LPS exhibited 
      some specific morphological features of typical apoptosis. the percentage of 
      apoptotic cells in HepG(2) cells treated with LPS and hrGH was significantly 
      lower than that treated with LPS (36+/-5.6)% vs (99+/-0.8)%, P<0.001). 
      CONCLUSIONS: LPS can induce apoptosis of HepG(2) cells, and hrGH can downregulate 
      the apoptotic role of LPS on HepG(2) cells.
FAU - Zhang, D
AU  - Zhang D
AD  - The Institute of Viral Hepatitis, Chongqing University of Medical Sciences, 
      Chongqing 400010, China.
FAU - Zhang, D
AU  - Zhang D
FAU - Ren, H
AU  - Ren H
FAU - Zhou, W
AU  - Zhou W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 12629-01-5 (Human Growth Hormone)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Down-Regulation
MH  - Hepatocytes/*drug effects
MH  - Human Growth Hormone/*pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Lipopolysaccharides/*pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2001/05/15 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/05/15 10:00
PHST- 2001/05/15 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/05/15 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2001 Apr;9(2):100-2.