PMID- 11351302
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 92
IP  - 6
DP  - 2001 Jun 15
TI  - Characterization of chromosomal abnormalities in uroepithelial carcinomas by 
      G-banding, spectral karyotyping and FISH analysis.
PG  - 824-31
AB  - Chromosome analysis by G-banding, spectral karyotyping (SKY) and fluorescence in 
      situ hybridization (FISH) was performed on 24 short-term cultured transitional 
      cell bladder carcinomas and 5 cell lines established from bladder carcinomas. 
      Except for one tumor with an apparently normal chromosomal constitution, clonal 
      chromosome abnormalities were detected in all examined cases by the combined 
      approach. The application of SKY and FISH techniques improved the karyotypic 
      descriptions, originally based on G-banding only, by identifying 32 additional 
      numerical changes, by establishing the chromosomal origin of 27 markers and 2 
      ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden 
      chromosomal rearrangements. No recurrent translocation, however, was detected. 
      The most prominent karyotypic feature was thus the occurrence of deletions and 
      losses of whole chromosome copies indicating the importance of tumor suppressor 
      genes in transitional cell carcinoma pathogenesis. Invasive carcinomas were 
      karyotypically more complex than were low grade superficial tumors. Specific 
      losses of material from chromosome 9 and from chromosome arms 11p and 8p, and 
      gains of 8q and 1q seem to be early changes appearing in superficial tumors, 
      whereas losses from 4p and 17p and the formation of an isochromosome for 5p were 
      associated with more aggressive tumor phenotypes.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Fadl-Elmula, I
AU  - Fadl-Elmula I
AD  - Department of Clinical Genetics, University Hospital, Lund, Sweden. 
      Imad.elmula@klingen.lu.se
FAU - Kytola, S
AU  - Kytola S
FAU - Pan, Y
AU  - Pan Y
FAU - Lui, W O
AU  - Lui WO
FAU - Derienzo, G
AU  - Derienzo G
FAU - Forsberg, L
AU  - Forsberg L
FAU - Mandahl, N
AU  - Mandahl N
FAU - Gorunova, L
AU  - Gorunova L
FAU - Bergerheim, U S
AU  - Bergerheim US
FAU - Heim, S
AU  - Heim S
FAU - Larsson, C
AU  - Larsson C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*genetics
MH  - Carcinoma, Transitional Cell/genetics
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Chromosome Deletion
MH  - *Chromosome Disorders
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 9
MH  - Epithelium/*pathology/ultrastructure
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Isochromosomes
MH  - *Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Models, Genetic
MH  - Neoplasms, Glandular and Epithelial/*genetics
MH  - Ring Chromosomes
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/*genetics/ultrastructure
EDAT- 2001/05/15 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/05/15 10:00
PHST- 2001/05/15 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/05/15 10:00 [entrez]
AID - 10.1002/ijc.1267 [pii]
AID - 10.1002/ijc.1267 [doi]
PST - ppublish
SO  - Int J Cancer. 2001 Jun 15;92(6):824-31. doi: 10.1002/ijc.1267.