PMID- 11352554
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20131121
IS  - 0016-6480 (Print)
IS  - 0016-6480 (Linking)
VI  - 122
IP  - 1
DP  - 2001 Apr
TI  - Insulin-like effect of zinc in mytilus digestive gland cells: modulation of 
      tyrosine kinase-mediated cell signaling.
PG  - 60-6
AB  - The possible effects of zinc in the modulation of the activity of glycolytic 
      enzymes phosphofructokinase and pyruvate kinase through tyrosine kinase-mediated 
      signal transduction in isolated digestive gland cells from mussels (Mytilus 
      galloprovincialis Lam.) were investigated. Addition of micromolar concentrations 
      of zinc resulted in both time- and concentration-dependent stimulation of 
      glycolytic enzyme activities similar to those previously observed with insulin; 
      however, zinc pretreatment prevented the glycolytic effect of insulin in mussel 
      cells. The insulin-like effect of zinc was mediated by increased tyrosine 
      phosphorylation of multiple proteins, as demonstrated by Western blotting with 
      antiphosphotyrosine antibodies. The pattern of zinc-induced phosphorylation 
      resembled that induced by insulin. Moreover, both zinc and insulin induced 
      activation of mitogen activated protein kinases (MAPKs); however, whereas zinc 
      gave a clear effect on the stress-activated p-38 MAPK, insulin activated 
      extracellular-activated MAPK (ERK2) and inhibited p-38. The results demonstrate 
      that zinc can act as a physiological regulator of tyrosine kinase-mediated cell 
      signaling in mussel digestive gland cells, in particular at the level of MAPK 
      activation. Activation of p-38 by zinc may be a key step in prevention of the 
      glycolytic effect of insulin in mussel cells. These data underline the importance 
      of cross talk between different MAPKs in determination of the response to 
      extracellular stimuli in marine invertebrate cells.
CI  - Copyright 2001 Academic Press.
FAU - Canesi, L
AU  - Canesi L
AD  - Istituto Scienze Fisiologiche, Universita di Urbino, Loc. Crocicchia, Urbino 
      (PU), 61029, Italy.
FAU - Betti, M
AU  - Betti M
FAU - Ciacci, C
AU  - Ciacci C
FAU - Gallo, G
AU  - Gallo G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Insulin)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Bivalvia/*metabolism
MH  - Blotting, Western
MH  - Digestion
MH  - Enzyme Activation/drug effects
MH  - Glycolysis/drug effects
MH  - Insulin/*pharmacology
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Phosphofructokinase-1/metabolism
MH  - Phosphorylation
MH  - Phosphotyrosine/analysis/metabolism
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Signal Transduction/*drug effects
MH  - Zinc/administration & dosage/*pharmacology
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2001/05/16 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/16 10:00
PHST- 2001/05/16 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/16 10:00 [entrez]
AID - S0016-6480(01)97612-9 [pii]
AID - 10.1006/gcen.2001.7612 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2001 Apr;122(1):60-6. doi: 10.1006/gcen.2001.7612.