PMID- 11352659
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 282
IP  - 4
DP  - 2001 Apr 13
TI  - New isoforms of VEGF are translated from alternative initiation CUG codons 
      located in its 5'UTR.
PG  - 1053-60
AB  - Vascular endothelial growth factor (VEGF) has a central role in normal as well as 
      in tumor angiogenesis. As such, VEGF is subjected to multi-level regulation at 
      the transcriptional, post-transcriptional, translational, and post-translational 
      levels to ensure proper expression during embryogenesis and adulthood. Its mRNA 
      contains an exceptionally long (1038 bp) 5' untranslated region (5'UTR), which 
      has a role in transcriptional as well as translational regulation of VEGF 
      expression. In this communication, we provide new evidence showing that an open 
      reading frame (ORF) present in the 5'UTR encodes for new putative isoforms of 
      VEGF due to alternative translational initiation from CUG codons. Like VEGF, the 
      translation of the new isoforms is not sensitive to stress signals such as 
      anoxia. Most likely, these isoforms either possess new capabilities, which are 
      different from the activity of the classical VEGF isoforms, or affect the 
      efficiency and capacity of translational initiation from the canonical AUG codon.
CI  - Copyright 2001 Academic Press.
FAU - Meiron, M
AU  - Meiron M
AD  - Technion, Department of Food Engineering and Biotechnology, Haifa, 32000, Israel.
FAU - Anunu, R
AU  - Anunu R
FAU - Scheinman, E J
AU  - Scheinman EJ
FAU - Hashmueli, S
AU  - Hashmueli S
FAU - Levi, B Z
AU  - Levi BZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antibodies)
RN  - 0 (Codon, Initiator)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Protein Isoforms)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Antibodies/immunology
MH  - COS Cells
MH  - *Codon, Initiator
MH  - Endothelial Growth Factors/*genetics/immunology
MH  - Humans
MH  - Lymphokines/*genetics/immunology
MH  - Molecular Weight
MH  - Mutagenesis, Site-Directed
MH  - Open Reading Frames
MH  - *Protein Biosynthesis
MH  - Protein Isoforms/genetics/immunology
MH  - Transfection
MH  - U937 Cells
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2001/05/16 10:00
MHDA- 2001/06/08 10:01
CRDT- 2001/05/16 10:00
PHST- 2001/05/16 10:00 [pubmed]
PHST- 2001/06/08 10:01 [medline]
PHST- 2001/05/16 10:00 [entrez]
AID - S0006-291X(01)94684-4 [pii]
AID - 10.1006/bbrc.2001.4684 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Apr 13;282(4):1053-60. doi: 
      10.1006/bbrc.2001.4684.