PMID- 11353289
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20190910
IS  - 0300-4864 (Print)
IS  - 0300-4864 (Linking)
VI  - 29
IP  - 9
DP  - 2000 Sep
TI  - Fibroblast-like cells from rat plantar skin and neurotrophin-transfected 3T3 
      fibroblasts influence neurite growth from rat sensory neurons in vitro.
PG  - 653-63
AB  - Our previous finding that skin-derived and muscle-derived molecules can be used 
      to sort regenerating rat sciatic nerve axons evoked questions concerning 
      neuron-target interactions at the level of single cells, which prompted the 
      present study. The results show that dorsal root ganglion (DRG) neurons 
      co-cultured with fibroblast-like skin-derived cells emit many neurites. These 
      have a proximal linear segment and a distal network of beaded branches in direct 
      relation to skin-derived cells. Electron microscopic examination of such 
      co-cultures showed bundles of neurites at some distance from the target cells and 
      single profiles closely apposed to subjacent cells. RNase protection assay 
      revealed that cultivated skin-derived cells express nerve growth factor (NGF), 
      brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and 
      neurotrophin-4 (NT-4). In co-cultures of DRG neurons and 3T3 fibroblasts 
      overexpressing either of the neurotrophins produced by skin-derived cells the 
      picture varied. NT-3 transfected 3T3 fibroblasts gave a growth pattern similar to 
      that seen with skin-derived cells. Neurons co-cultured with mock-transfected 3T3 
      fibroblasts were small and showed weak neurite growth. In co-cultures with a 
      membrane insert between skin-derived cells or 3T3 fibroblasts and DRG neurons few 
      neurons survived and neurite growth was very sparse. We conclude that 
      skin-derived cells stimulate neurite growth from sensory neurons in vitro, that 
      these cells produce NGF, BDNF, NT-3 and NT-4 and that 3T3 fibroblasts producing 
      NT-3 mimic the effect of skin-derived cells on sensory neurons in co-culture. 
      Finally the results suggest that cell surface molecules are important for 
      neuritogenesis.
FAU - Jerregard, H
AU  - Jerregard H
AD  - Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of 
      Health Sciences, University of Linkoping, Linkoping, Sweden.
FAU - Akerud, P
AU  - Akerud P
FAU - Arenas, E
AU  - Arenas E
FAU - Hildebrand, C
AU  - Hildebrand C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurocytol
JT  - Journal of neurocytology
JID - 0364620
RN  - 0 (Gold Compounds)
RN  - 0 (Nerve Growth Factors)
RN  - 68238-35-7 (Keratins)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - 3T3 Cells/cytology/*transplantation
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Communication/*genetics
MH  - Cell Differentiation/physiology
MH  - Cell Size/physiology
MH  - Cells, Cultured/cytology/*transplantation
MH  - Coculture Techniques/methods
MH  - Epidermal Cells
MH  - Epidermis/metabolism/transplantation
MH  - Ganglia, Spinal/cytology/*growth & development/metabolism
MH  - Gold Compounds/pharmacokinetics
MH  - Immunohistochemistry
MH  - Keratins/metabolism
MH  - Mice
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Nerve Regeneration/genetics
MH  - Neurites/*metabolism/ultrastructure
MH  - Neurons, Afferent/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thiolester Hydrolases/metabolism
MH  - Tissue Transplantation
MH  - Transfection
MH  - Ubiquitin Thiolesterase
EDAT- 2001/05/16 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/05/16 10:00
PHST- 2001/05/16 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/05/16 10:00 [entrez]
AID - 10.1023/a:1010883320683 [doi]
PST - ppublish
SO  - J Neurocytol. 2000 Sep;29(9):653-63. doi: 10.1023/a:1010883320683.