PMID- 11356643
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20200930
IS  - 0363-6135 (Print)
IS  - 0363-6135 (Linking)
VI  - 280
IP  - 6
DP  - 2001 Jun
TI  - Homocysteine induces monocyte chemoattractant protein-1 expression by activating 
      NF-kappaB in THP-1 macrophages.
PG  - H2840-7
AB  - Homocysteinemia is an independent risk factor for cardiovascular disorders. The 
      recruitment of monocytes is an important event in atherogenesis. Monocyte 
      chemoattractant protein-1 (MCP-1) is a potent chemokine that stimulates monocyte 
      migration into the intima of arterial walls. The objective of the present study 
      was to investigate the effect of homocysteine on MCP-1 expression in macrophages 
      and the underlying mechanism of such effect. Human monocytic cell (THP-1)-derived 
      macrophages were incubated with homocysteine. By nuclease protection assay and 
      ELISA, homocysteine (0.05-0.2 mM) was shown to significantly enhance the 
      expression of MCP-1 mRNA (up to 2.6-fold) and protein (up to 4.8-fold) in these 
      cells. Homocysteine-induced MCP-1 expression resulted in increased monocyte 
      chemotaxis. The increase in MCP-1 expression was associated with activation of 
      nuclear factor (NF)-kappaB due to increased phosphorylation of the inhibitory 
      protein (IkappaB-alpha) as well as reduced expression of IkappaB-alpha mRNA in 
      homocysteine-treated cells. In conclusion, our results demonstrate that 
      homocysteine, at pathological concentration, stimulates MCP-1 expression in THP-1 
      macrophages via NF-kappaB activation.
FAU - Wang, G
AU  - Wang G
AD  - Department of Pharmacology and Institute of Cardiovascular Science and Medicine, 
      Faculty of Medicine, University of Hong Kong, Hong Kong, China.
FAU - Siow, Y L
AU  - Siow YL
FAU - O, K
AU  - O K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal, 28S)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 2104-86-1 (Tosyllysine Chloromethyl Ketone)
RN  - 9007-49-2 (DNA)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Antibodies/pharmacology
MH  - Binding, Competitive/drug effects
MH  - Cell Line
MH  - Chemokine CCL2/antagonists & inhibitors/*biosynthesis/genetics
MH  - Chemotaxis/drug effects
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression/drug effects
MH  - Homocysteine/*pharmacology
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Macrophages/cytology/*drug effects/*metabolism
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/antagonists & inhibitors/*metabolism
MH  - RNA, Messenger/metabolism
MH  - RNA, Ribosomal, 28S/metabolism
MH  - Tosyllysine Chloromethyl Ketone/pharmacology
EDAT- 2001/05/18 10:00
MHDA- 2001/06/23 10:01
CRDT- 2001/05/18 10:00
PHST- 2001/05/18 10:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/05/18 10:00 [entrez]
AID - 10.1152/ajpheart.2001.280.6.H2840 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2001 Jun;280(6):H2840-7. doi: 
      10.1152/ajpheart.2001.280.6.H2840.