PMID- 11356880 OWN - NLM STAT- MEDLINE DCOM- 20010621 LR - 20191023 IS - 1529-2401 (Electronic) IS - 0270-6474 (Print) IS - 0270-6474 (Linking) VI - 21 IP - 11 DP - 2001 Jun 1 TI - Effects of early visual experience and diurnal rhythms on BDNF mRNA and protein levels in the visual system, hippocampus, and cerebellum. PG - 3923-31 AB - The expression of brain-derived neurotrophic factor (BDNF) mRNA and the secretion of BDNF protein are tightly regulated by neuronal activity. Thus, BDNF has been proposed as a mediator of activity-dependent neural plasticity. Previous studies showed that dark rearing (DR) reduces BDNF mRNA levels in the primary visual cortex (V1), but the effects of visual experience on BDNF protein levels are unknown. We report that rearing in constant light or DR alters BDNF mRNA and protein levels in the retina, superior colliculus (SC), V1, hippocampus (HIPP), and cerebellum (CBL), although the changes in mRNA and protein are not always correlated. Most notably, DR increases BDNF protein levels in V1 although BDNF mRNA is decreased. BDNF protein levels also undergo diurnal changes. In the retina, V1, and SC, BDNF protein levels are higher during the light phase of the circadian cycle than during the dark phase. By contrast, in HIPP and CBL, the tissue concentration of BDNF protein is higher during the dark phase. The discrepancies between the experience-dependent changes in BDNF mRNA and protein suggest that via its effects on neuronal activity, early sensory experience alters the trafficking, as well as the synthesis, of BDNF protein. The circadian changes in BDNF protein suggest that BDNF could cause the diurnal modulation of synaptic efficacy in some neural circuits. The fluctuations in BDNF levels in nonvisual structures suggest a potential role of BDNF in mediating plasticity induced by hormones or motor activity. FAU - Pollock, G S AU - Pollock GS AD - Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. FAU - Vernon, E AU - Vernon E FAU - Forbes, M E AU - Forbes ME FAU - Yan, Q AU - Yan Q FAU - Ma, Y T AU - Ma YT FAU - Hsieh, T AU - Hsieh T FAU - Robichon, R AU - Robichon R FAU - Frost, D O AU - Frost DO FAU - Johnson, J E AU - Johnson JE LA - eng GR - NS07375/NS/NINDS NIH HHS/United States GR - EY11434/EY/NEI NIH HHS/United States GR - MH49568/MH/NIMH NIH HHS/United States GR - T32 NS007375/NS/NINDS NIH HHS/United States GR - EY11127/EY/NEI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) SB - IM MH - Aging/metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cerebellum/*metabolism MH - Circadian Rhythm/*physiology MH - Cricetinae MH - Darkness MH - Hippocampus/*metabolism MH - Light MH - Mesocricetus MH - Neuronal Plasticity/physiology MH - Photic Stimulation/methods MH - RNA, Messenger/*metabolism MH - Rats MH - Rats, Long-Evans MH - Retina/metabolism MH - Superior Colliculi/metabolism MH - Visual Cortex/metabolism MH - Visual Pathways/metabolism PMC - PMC6762725 EDAT- 2001/05/23 10:00 MHDA- 2001/06/22 10:01 PMCR- 2001/12/01 CRDT- 2001/05/23 10:00 PHST- 2001/05/23 10:00 [pubmed] PHST- 2001/06/22 10:01 [medline] PHST- 2001/05/23 10:00 [entrez] PHST- 2001/12/01 00:00 [pmc-release] AID - 21/11/3923 [pii] AID - 5262 [pii] AID - 10.1523/JNEUROSCI.21-11-03923.2001 [doi] PST - ppublish SO - J Neurosci. 2001 Jun 1;21(11):3923-31. doi: 10.1523/JNEUROSCI.21-11-03923.2001.