PMID- 11357512
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20190906
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 175
IP  - 3
DP  - 2001 Mar
TI  - Enrichment and characterization of an anammox bacterium from a rotating 
      biological contactor treating ammonium-rich leachate.
PG  - 198-207
AB  - Anaerobic ammonium oxidation with nitrite to N2 (anammox) is a recently 
      discovered microbial reaction with interesting potential for nitrogen removal 
      from wastewater. We enriched an anammox culture from a rotating disk contactor 
      (near Kolliken, Switzerland) that was used to treat ammonium-rich leachate with 
      low organic carbon content. This enrichment led to a relative population size of 
      88% anammox bacteria. The microorganism carrying out the anammox reaction was 
      identified by analysis of the 16S rDNA sequence and by fluorescence in situ 
      hybridization (FISH) with 16S-rRNA-targeting probes. The percentage sequence 
      identity between the 16S rDNA sequences of the Kolliken anammox organism and the 
      archetype anammox strain Candidatus Brocadia anammoxidans was 90.9%, but between 
      98.5 and 98.9% with Candidatus Kuenenia stuttgartiensis, an organism identified 
      in biofilms by molecular methods. The Kolliken culture catalyzed the anaerobic 
      oxidation of ammonium with nitrite in a manner seemingly identical to that of 
      Candidatus B. anammoxidans, but exhibited higher tolerance to phosphate (up to 20 
      mM) and to nitrite (up to 13 mM) and was active at lower cell densities. Anammox 
      activity was observed only between pH 6.5 and 9, with an optimum at pH 8 and a 
      temperature optimum at 37 degrees C. Hydroxylamine and hydrazine, which are 
      intermediates of the anammox reaction of Candidatus B. anammoxidans, were 
      utilized by the Kolliken organisms, and approximately 15% of the nitrite utilized 
      during autotrophic growth was converted to nitrate. Electron microscopy showed a 
      protein-rich region in the center of the cells surrounded by a doughnut-shaped 
      region containing ribosomes and DNA. This doughnut-shape region was observed with 
      FISH as having a higher fluorescence intensity. Similar to Candidatus B. 
      anammoxidans, the Kolliken anammox organism typically formed homogenous clusters 
      containing up to several hundred cells within an extracellular matrix.
FAU - Egli, K
AU  - Egli K
AD  - Eidgenossische Anstalt fur Wasserversorgung, Abwasserreinigung und Gewasserschutz 
      (EAWAG), Uberlandstrasse 133, Postfach 611, 8600 Dubendorf, Switzerland.
FAU - Fanger, U
AU  - Fanger U
FAU - Alvarez, P J
AU  - Alvarez PJ
FAU - Siegrist, H
AU  - Siegrist H
FAU - van der Meer, J R
AU  - van der Meer JR
FAU - Zehnder, A J
AU  - Zehnder AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Nitrites)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria/*metabolism
MH  - Biofilms
MH  - Hydrogen-Ion Concentration
MH  - Nitrites/*metabolism
MH  - Nitrogen/*metabolism
MH  - Oxidation-Reduction
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - Temperature
EDAT- 2001/05/19 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/05/19 10:00
PHST- 2001/05/19 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/05/19 10:00 [entrez]
AID - 10.1007/s002030100255 [doi]
PST - ppublish
SO  - Arch Microbiol. 2001 Mar;175(3):198-207. doi: 10.1007/s002030100255.