PMID- 11357878
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20191104
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 1
IP  - 4
DP  - 2001 Apr
TI  - Vaccination with MCP-1 cDNA transfectant on human malignant glioma in nude mice 
      induces migration of monocytes and NK cells to the tumor.
PG  - 657-64
AB  - Recently, studies on vaccination with tumor cells genetically engineered to 
      produce monocyte chemoattractant protein-1 (MCP-1) have provided some 
      encouragement. These studies have shown infiltration of certain types of 
      lymphocytes at the tumor site. However, natural killer (NK) cells have not yet 
      been assessed. We obtained a human malignant glioma cell line producing human 
      MCP-1 constitutively by transfection of MCP-1 cDNA. We then test the effect of 
      vaccination with the MCP-1 transfectant on nude mice. Although vaccination with 
      MCP-1 transfectant did not reduce the tumor in our study, it was associated with 
      the infiltration of large numbers of NK cells and monocytes at the tumor site. 
      The site of vaccination also showed large numbers of monocytes. NK cells were 
      detected with anti-asialo GM1 antibody, and monocytes were detected 
      immunohistochemically with F4/80. We assumed that infiltrating monocytes at the 
      site of vaccination could promote the infiltration of monocytes and NK cells into 
      the tumor site without T-cell mediated transduction because the host lacked 
      T-cell function.
FAU - Nagai, M
AU  - Nagai M
AD  - Department of Surgical Neurology, Jichi Medical School, Tochigi, Japan. 
      mnagai@jichi.ac.jp
FAU - Masuzawa, T
AU  - Masuzawa T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Cancer Vaccines)
RN  - 0 (Chemokine CCL2)
RN  - 37758-47-7 (G(M1) Ganglioside)
SB  - IM
MH  - Animals
MH  - Cancer Vaccines/*immunology
MH  - Cell Movement
MH  - Chemokine CCL2/genetics/*physiology
MH  - G(M1) Ganglioside/analysis
MH  - Glioma/*immunology/pathology
MH  - Humans
MH  - Killer Cells, Natural/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Monocytes/*physiology
MH  - Transfection
MH  - Vaccination
EDAT- 2001/05/19 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/05/19 10:00
PHST- 2001/05/19 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/05/19 10:00 [entrez]
AID - S1567-5769(00)00050-3 [pii]
AID - 10.1016/s1567-5769(00)00050-3 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2001 Apr;1(4):657-64. doi: 10.1016/s1567-5769(00)00050-3.