PMID- 11357890
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20191104
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 1
IP  - 4
DP  - 2001 Apr
TI  - Capsazepine, a vanilloid receptor antagonist, inhibits the expression of 
      inducible nitric oxide synthase gene in lipopolysaccharide-stimulated RAW264.7 
      macrophages through the inactivation of nuclear transcription factor-kappa B.
PG  - 777-84
AB  - High amounts of nitric oxide (NO) production following the induction of inducible 
      NO synthase (iNOS) gene expression has been implicated in the pathogenesis of 
      inflammatory diseases. Capsaicin, a vanilloid receptor agonist, is known to have 
      an inhibitory effect on NO production in macrophages. In the present study, we 
      have found that capsazepine (CAPZ), a vanilloid receptor antagonist, also 
      inhibited NO and iNOS protein syntheses induced by lipopolysaccharide in RAW264.7 
      macrophages via the suppression of iNOS mRNA. The mechanistic studies showed that 
      CAPZ inhibited the expression of iNOS mRNA through the inactivation of nuclear 
      transcription factor-kappa B (NF-kappa B). Thus, capsazepine may be a useful 
      candidate for the development of a drug to treat inflammatory diseases related to 
      iNOS gene overexpression.
FAU - Oh, G S
AU  - Oh GS
AD  - Medicinal Resources Research Center (MRRC), Wonkwang University, Chonbuk, South 
      Korea.
FAU - Pae, H O
AU  - Pae HO
FAU - Seo, W G
AU  - Seo WG
FAU - Kim, N Y
AU  - Kim NY
FAU - Pyun, K H
AU  - Pyun KH
FAU - Kim, I K
AU  - Kim IK
FAU - Shin, M
AU  - Shin M
FAU - Chung, H T
AU  - Chung HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Drug)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - LFW48MY844 (capsazepine)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Capsaicin/*analogs & derivatives/*pharmacology
MH  - Cell Line
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/*enzymology
MH  - Mice
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Nitric Oxide/biosynthesis
MH  - Nitric Oxide Synthase/*antagonists & inhibitors/genetics
MH  - Nitric Oxide Synthase Type II
MH  - RNA, Messenger/analysis
MH  - Receptors, Drug/*antagonists & inhibitors
EDAT- 2001/05/19 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/05/19 10:00
PHST- 2001/05/19 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/05/19 10:00 [entrez]
AID - S1567576901000121 [pii]
AID - 10.1016/s1567-5769(01)00012-1 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2001 Apr;1(4):777-84. doi: 10.1016/s1567-5769(01)00012-1.