PMID- 11359446
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20230412
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 124
IP  - 1
DP  - 2001 Apr
TI  - Dipalmitoylphosphatidylcholine modulates inflammatory functions of monocytic 
      cells independently of mitogen activated protein kinases.
PG  - 86-94
AB  - Phosphatidylcholine (PC) is the major phospholipid of pulmonary surfactant and it 
      is hypothesized that PC and its subspecies modulate the functions of alveolar 
      macrophages. The most abundant of these subspecies is 
      dipalmitoylphosphatidylcholine (DPPC). This study was undertaken to determine the 
      effect of PC on monocyte function using a human monocytic cell line, MonoMac-6 
      (MM6). This study showed that preincubation of MM6 cells with DPPC at 125 
      microg/ml for 2 h inhibited the oxidative response to either zymosan or 
      phorbol-12-myristate-13-acetate (PMA) by 30% (P < 0.001). This inhibition with 
      DPPC was independent of LPS priming. When DPPC was replaced with 
      1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC) there was no inhibition and 
      in contrast a significant increase in oxidant production was observed. We also 
      demonstrated that total PC (tPC; a heterogeneous species of PC from egg) and DPPC 
      but not PAPC significantly inhibited the release of TNF-alpha from MM6 cells (P < 
      0.05). DPPC did not inhibit phosphorylation of the mitogen activated protein 
      kinases (MAPKs) p44/p42 or p38 in stimulated cells. Measurements of membrane 
      fluidity with spin label EPR spectroscopy indicate that DPPC incorporation 
      significantly alters the membrane fluidity of MM6 cells. These results suggest 
      that DPPC, the major component of pulmonary surfactant, may play a role in 
      modulating leucocyte inflammatory responses in the lung. This may in part be 
      related to membrane effects but does not include alterations in p44/p42 or p38 
      MAPK signalling.
FAU - Tonks, A
AU  - Tonks A
AD  - School of Applied Sciences, University of Wales Institute, Cardiff, UK.
FAU - Morris, R H
AU  - Morris RH
FAU - Price, A J
AU  - Price AJ
FAU - Thomas, A W
AU  - Thomas AW
FAU - Jones, K P
AU  - Jones KP
FAU - Jackson, S K
AU  - Jackson SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Membrane Lipids)
RN  - 0 (Phospholipids)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 2644-64-6 (1,2-Dipalmitoylphosphatidylcholine)
RN  - 9010-72-4 (Zymosan)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 1,2-Dipalmitoylphosphatidylcholine/metabolism/*pharmacology
MH  - Cell Line
MH  - Humans
MH  - Inflammation
MH  - Luminescent Measurements
MH  - *MAP Kinase Signaling System
MH  - Membrane Lipids/metabolism
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Monocytes/*drug effects/metabolism
MH  - Phospholipids/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Processing, Post-Translational/drug effects
MH  - Pulmonary Surfactants/physiology
MH  - Reactive Oxygen Species/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Zymosan/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases
PMC - PMC1906030
EDAT- 2001/05/22 10:00
MHDA- 2001/06/23 10:01
CRDT- 2001/05/22 10:00
PHST- 2001/05/22 10:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/05/22 10:00 [entrez]
AID - cei1479 [pii]
AID - 10.1046/j.1365-2249.2001.01479.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2001 Apr;124(1):86-94. doi: 10.1046/j.1365-2249.2001.01479.x.