PMID- 11359653
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20091119
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 4
DP  - 2001 Apr
TI  - Monocyte chemoattractant protein-1 production by intestinal epithelial cells in 
      vitro: a role for p38 in epithelial chemokine expression.
PG  - 223-30
AB  - The intestinal epithelial cell (IEC) represents the first cellular barrier to 
      infection. Consistent with this sentinel role, IEC are known to produce a variety 
      of chemokines in response to bacterial infection or proinflammatory cytokines. 
      These chemokines act as potent leukocyte activators and chemoattractants in vivo. 
      In this report, we begin to characterize the regulation of expression of the 
      chemokine monocyte chemoattractant protein-1 (MCP-1) in the rat small intestinal 
      IEC-18 line. Following stimulation with either interleukin-1beta (IL-1beta) or 
      lipopolysaccharide (LPS), IEC-18 cells produced MCP-1, with IL-1 proving a more 
      effective stimulus than LPS at both the mRNA and protein levels. Expression of 
      MCP-1 due to either stimulus was inhibited by tyrosine kinase inhibitors, 
      prompting us to investigate potential phosphotyrosine-dependent targets 
      responsible for MCP-1 expression. We detected activation of p38, a member of the 
      mitogen-activated protein kinase family, following either IL-1 or LPS treatment. 
      Specific inhibition of this kinase using the compound SB203580 caused a 
      destabilization of MCP-1 mRNA. These data point to a role for p38 in the 
      regulation of MCP-1 mRNA expression by the IEC.
FAU - Waterhouse, C C
AU  - Waterhouse CC
AD  - Department of Microbiology and Immunology, Dalhousie University, Halifax, NS 
      Canada.
FAU - Joseph, R R
AU  - Joseph RR
FAU - Winsor, G L
AU  - Winsor GL
FAU - Lacombe, T A
AU  - Lacombe TA
FAU - Stadnyk, A W
AU  - Stadnyk AW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/antagonists & inhibitors/*biosynthesis/genetics
MH  - Chemokines/*biosynthesis
MH  - Down-Regulation/immunology
MH  - Enzyme Activation/immunology
MH  - Enzyme Inhibitors/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Intestinal Mucosa/*immunology/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*physiology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/pharmacology
MH  - RNA Stability/immunology
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Rats
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2001/05/22 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/05/22 10:00
PHST- 2001/05/22 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/05/22 10:00 [entrez]
AID - 10.1089/107999001750169853 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Apr;21(4):223-30. doi: 
      10.1089/107999001750169853.